Effect of breviscapine against hepatic ischemia reperfusion injury

Abstract Background Breviscapine is an active ingredient extracted from traditional Chinese medicine Erigeron breviscapus . The purpose of this study was to investigate the effect of breviscapine injection on hepatic ischemia and/or reperfusion injury. Methods Forty rats were randomly divided into f...

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Bibliographic Details
Published in:The Journal of surgical research 2016-06, Vol.203 (2), p.268-274
Main Authors: Lin, Yan-zhu, Lu, Zhi-yuan, Liang, Xiao-hui, Li, Kang, Peng, Bo, Gong, Jin
Format: Article
Language:English
Subjects:
Animals
Biomarkers - metabolism
Blotting, Western
Breviscapine
Drug Administration Schedule
Drugs, Chinese Herbal - pharmacology
Drugs, Chinese Herbal - therapeutic use
Erigeron breviscapus
Flavonoids - pharmacology
Flavonoids - therapeutic use
Injections, Intravenous
Ischemia reperfusion
Liver
Liver - drug effects
Liver - metabolism
Liver - pathology
Male
Mitofusin 2
Oxidative Stress - drug effects
Protective Agents - pharmacology
Protective Agents - therapeutic use
Random Allocation
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Surgery
Treatment Outcome
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Summary:Abstract Background Breviscapine is an active ingredient extracted from traditional Chinese medicine Erigeron breviscapus . The purpose of this study was to investigate the effect of breviscapine injection on hepatic ischemia and/or reperfusion injury. Methods Forty rats were randomly divided into five groups ( n  = 8): Sham group, Ischemia reperfusion 1 (I/R1) + normal saline (NS) group, I/R1 + breviscapine (Bre), I/R2 + NS group, and I/R2 + Bre group. Group1 and group2 represent ischemia time for 10 min and 30 min, respectively. Breviscapine or normal saline was administered to rats (single dose of 10 mg/Kg, intravenously) 30 min before hepatic ischemia. Serum transaminases, histopathologic changes, malondialdehyde (MDA), and superoxide dismutase (SOD) in liver tissues were evaluated. The expression of mitochondrial fusion 2 (Mfn2) was also investigated. Results After 24-h reperfusion, based on the histopathologic analysis, compared with NS control group, the liver function was improved in breviscapine group. Liver enzymes aspartate and alanine aminotransferase levels were significantly lower in the I/R + Bre group, when compared with the I/R + NS group. Pretreatment with breviscapine reduced MDA level ( P  
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2016.02.013