Epiphytic yeast's kinetics characterization and storage decay biocontrol on apple fruit

Fruit naturally occurring yeast have been targeted as potential antagonist to postharvest fungal diseases, since they exhibit several features that confer them greater potential for colonizing fruit surfaces and wound sites. Commercial use of these biocontrol agents is still very limited and account...

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Bibliographic Details
Published in:Phytopathology 2004-06, Vol.94 (6)
Main Authors: Martinez-Campos, A R, Guerrero-Prieto, V M
Format: Article
Language:English
Subjects:
Botrytis cinerea
Candida oleophila
Malus domestica
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Summary:Fruit naturally occurring yeast have been targeted as potential antagonist to postharvest fungal diseases, since they exhibit several features that confer them greater potential for colonizing fruit surfaces and wound sites. Commercial use of these biocontrol agents is still very limited and accounts for only a very small fraction of their potential market. Understanding yeast's mode of action, is a must for developing successful biocontrol strategies. Three strains of Candida oleophila, isolated from apple fruit were tested for their control ability of storage decay on apples and their specific growth and consumption rate were determined. Parameters were established by batch growth in 2 1 bioreactors. For inoculation experiment, yeast cells were cultured and harvested at exponential growth phase. Strain identified as L06, inhibited 62% of Botrytis cinerea growth, while L07, nonrugose (L) and rugose I strains only reduced 25% growth. Specific growth and consumption rates were; 0.25 and 0.19; 0.31 and 0.207; and 0.39, h super(-1) and g biomass/g glucose, for L06, L07L, and L07R, respectively. Results showed that lower specific growth rate increased yeast biocontrol activity, probably due to energy sources being used for lytic enzymes production.
ISSN:0031-949X