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Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth
Aim To evaluate the cytotoxicity, oxidative stress and genotoxicity in vitro of four iodoform pastes and three calcium hydroxide pastes. Methodology Peripheral blood mononuclear cells (PBMCs) and pure calf thymus DNA (dsDNA) were exposed to extracts of the pastes. Cytotoxicity was assessed with the...
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| Published in: | International endodontic journal 2016-08, Vol.49 (8), p.737-745 |
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| Main Authors: | , , , , , , , |
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| container_end_page | 745 |
| container_issue | 8 |
| container_start_page | 737 |
| container_title | International endodontic journal |
| container_volume | 49 |
| creator | Pires, C. W. Botton, G. Cadoná, F. C. Machado, A. K. Azzolin, V. F. da Cruz, I. B. M. Sagrillo, M. R. Praetzel, J. R. |
| description | Aim
To evaluate the cytotoxicity, oxidative stress and genotoxicity in vitro of four iodoform pastes and three calcium hydroxide pastes.
Methodology
Peripheral blood mononuclear cells (PBMCs) and pure calf thymus DNA (dsDNA) were exposed to extracts of the pastes. Cytotoxicity was assessed with the MTT assay. Generation of reactive oxygen species (ROS) was evaluated using a DCFH‐DA assay, and lipid peroxidation was evaluated using a TBARS assay. Genotoxicity was evaluated using the alkaline comet assay and Genomodifier capacity assay (GEMO). All tests were performed after 24 h and 72 h of cell exposure, except GEMO. After performing the Kolmogorov–Smirnov test, data were analysed by Kruskal–Wallis and Dunn's post‐tests, and anova with Dunnett's post‐test, with a significance level established at P |
| doi_str_mv | 10.1111/iej.12502 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1803793512</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1803793512</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5032-dcd466907f4b4b66064f3d1ca43e147fbf367e27a2972c9d2247e502fd5e8ed53</originalsourceid><addsrcrecordid>eNp1kE1v1DAQhi0EokvhwB9APoJEWn97c0RVWxZVRUit2pvl2OPiko2X2Gmbf49ht3tjLjOHZ17NPAi9p-SI1jqOcH9EmSTsBVpQrmTDZEtfogWhgjdsuZQH6E3O94QQSTh9jQ6YoloI3S5QXA1-ciWmAaeA3VxSSU_RxTJ_xnXwtsQHwLmMkDO2g8d3MOwR3M14TKngEPs-Dnd4Y3OBjKcMHscBb8a4tuOMC0D5-Ra9CrbP8G7XD9H12enVydfm4vv56uTLRePqcazxzgulWqKD6ESnFFEicE-dFRyo0KELXGlg2rJWM9d6xoSG-nrwEpbgJT9EH7e5mzH9niAXs47ZQd_bAdKUDV0SrlsuKavopy3qxpTzCMHsLjaUmL9mTTVr_pmt7Idd7NStwe_JZ5UVON4Cj7GH-f9JZnX67Tmy2W7Eau1pv2HHX0ZprqW5uTw34sctu7xqz8wN_wNT2pMc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1803793512</pqid></control><display><type>article</type><title>Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Pires, C. W. ; Botton, G. ; Cadoná, F. C. ; Machado, A. K. ; Azzolin, V. F. ; da Cruz, I. B. M. ; Sagrillo, M. R. ; Praetzel, J. R.</creator><creatorcontrib>Pires, C. W. ; Botton, G. ; Cadoná, F. C. ; Machado, A. K. ; Azzolin, V. F. ; da Cruz, I. B. M. ; Sagrillo, M. R. ; Praetzel, J. R.</creatorcontrib><description><![CDATA[Aim
To evaluate the cytotoxicity, oxidative stress and genotoxicity in vitro of four iodoform pastes and three calcium hydroxide pastes.
Methodology
Peripheral blood mononuclear cells (PBMCs) and pure calf thymus DNA (dsDNA) were exposed to extracts of the pastes. Cytotoxicity was assessed with the MTT assay. Generation of reactive oxygen species (ROS) was evaluated using a DCFH‐DA assay, and lipid peroxidation was evaluated using a TBARS assay. Genotoxicity was evaluated using the alkaline comet assay and Genomodifier capacity assay (GEMO). All tests were performed after 24 h and 72 h of cell exposure, except GEMO. After performing the Kolmogorov–Smirnov test, data were analysed by Kruskal–Wallis and Dunn's post‐tests, and anova with Dunnett's post‐test, with a significance level established at P < 0.05.
Results
The MTT assay revealed that chlorhexidine, Maxitrol and neomycin sulphate + bacitracin pastes decreased cell viability after 24 h (P < 0.05). No group was associated with a significant decreased cell viability or lipid peroxidation after 72 h. Calcium hydroxide pastes increased the cell viability levels at both experimental times (P < 0.05). Lipid peroxidation was observed with the exposure of cells to calcium hydroxide pastes after 24 h (P < 0.05). Exposure to chlorhexidine, Guedes‐Pinto and calcium hydroxide pastes resulted in a significant increase in ROS after 24 h (P < 0.05), whereas iodoform pastes and Calen thickened with zinc oxide significantly increased the ROS after 72 h (P < 0.05). The comet assay revealed that exposure of the PBMCs to iodoform pastes did not damage DNA at either period of time (P > 0.05). However, chlorhexidine paste caused DNA damage in dsDNA (P < 0.05). Calcium hydroxide pastes caused DNA damage in both tests (P < 0.05).
Conclusion
The pastes varied in their ability to induce cytotoxicity, genotoxicity and oxidative stress. In general, Guedes‐Pinto, Maxitrol and neomycin sulphate + bacitracin pastes exhibited better biocompatibility in vitro.]]></description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.12502</identifier><identifier>PMID: 26174479</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Analysis of Variance ; Animals ; biocompatibility ; calcium hydroxide ; Cattle ; deciduous tooth ; Dentistry ; DNA - drug effects ; DNA Damage ; Humans ; Hydrocarbons, Iodinated - pharmacology ; iodoform ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - metabolism ; Materials Testing ; Oxidative Stress - drug effects ; pulpectomy ; Reactive Oxygen Species - metabolism ; root canal filling materials ; Root Canal Filling Materials - pharmacology ; Statistics, Nonparametric ; Tooth, Deciduous ; Toxicity Tests</subject><ispartof>International endodontic journal, 2016-08, Vol.49 (8), p.737-745</ispartof><rights>2015 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2015 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5032-dcd466907f4b4b66064f3d1ca43e147fbf367e27a2972c9d2247e502fd5e8ed53</citedby><cites>FETCH-LOGICAL-c5032-dcd466907f4b4b66064f3d1ca43e147fbf367e27a2972c9d2247e502fd5e8ed53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26174479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pires, C. W.</creatorcontrib><creatorcontrib>Botton, G.</creatorcontrib><creatorcontrib>Cadoná, F. C.</creatorcontrib><creatorcontrib>Machado, A. K.</creatorcontrib><creatorcontrib>Azzolin, V. F.</creatorcontrib><creatorcontrib>da Cruz, I. B. M.</creatorcontrib><creatorcontrib>Sagrillo, M. R.</creatorcontrib><creatorcontrib>Praetzel, J. R.</creatorcontrib><title>Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description><![CDATA[Aim
To evaluate the cytotoxicity, oxidative stress and genotoxicity in vitro of four iodoform pastes and three calcium hydroxide pastes.
Methodology
Peripheral blood mononuclear cells (PBMCs) and pure calf thymus DNA (dsDNA) were exposed to extracts of the pastes. Cytotoxicity was assessed with the MTT assay. Generation of reactive oxygen species (ROS) was evaluated using a DCFH‐DA assay, and lipid peroxidation was evaluated using a TBARS assay. Genotoxicity was evaluated using the alkaline comet assay and Genomodifier capacity assay (GEMO). All tests were performed after 24 h and 72 h of cell exposure, except GEMO. After performing the Kolmogorov–Smirnov test, data were analysed by Kruskal–Wallis and Dunn's post‐tests, and anova with Dunnett's post‐test, with a significance level established at P < 0.05.
Results
The MTT assay revealed that chlorhexidine, Maxitrol and neomycin sulphate + bacitracin pastes decreased cell viability after 24 h (P < 0.05). No group was associated with a significant decreased cell viability or lipid peroxidation after 72 h. Calcium hydroxide pastes increased the cell viability levels at both experimental times (P < 0.05). Lipid peroxidation was observed with the exposure of cells to calcium hydroxide pastes after 24 h (P < 0.05). Exposure to chlorhexidine, Guedes‐Pinto and calcium hydroxide pastes resulted in a significant increase in ROS after 24 h (P < 0.05), whereas iodoform pastes and Calen thickened with zinc oxide significantly increased the ROS after 72 h (P < 0.05). The comet assay revealed that exposure of the PBMCs to iodoform pastes did not damage DNA at either period of time (P > 0.05). However, chlorhexidine paste caused DNA damage in dsDNA (P < 0.05). Calcium hydroxide pastes caused DNA damage in both tests (P < 0.05).
Conclusion
The pastes varied in their ability to induce cytotoxicity, genotoxicity and oxidative stress. In general, Guedes‐Pinto, Maxitrol and neomycin sulphate + bacitracin pastes exhibited better biocompatibility in vitro.]]></description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>biocompatibility</subject><subject>calcium hydroxide</subject><subject>Cattle</subject><subject>deciduous tooth</subject><subject>Dentistry</subject><subject>DNA - drug effects</subject><subject>DNA Damage</subject><subject>Humans</subject><subject>Hydrocarbons, Iodinated - pharmacology</subject><subject>iodoform</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Materials Testing</subject><subject>Oxidative Stress - drug effects</subject><subject>pulpectomy</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>root canal filling materials</subject><subject>Root Canal Filling Materials - pharmacology</subject><subject>Statistics, Nonparametric</subject><subject>Tooth, Deciduous</subject><subject>Toxicity Tests</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kE1v1DAQhi0EokvhwB9APoJEWn97c0RVWxZVRUit2pvl2OPiko2X2Gmbf49ht3tjLjOHZ17NPAi9p-SI1jqOcH9EmSTsBVpQrmTDZEtfogWhgjdsuZQH6E3O94QQSTh9jQ6YoloI3S5QXA1-ciWmAaeA3VxSSU_RxTJ_xnXwtsQHwLmMkDO2g8d3MOwR3M14TKngEPs-Dnd4Y3OBjKcMHscBb8a4tuOMC0D5-Ra9CrbP8G7XD9H12enVydfm4vv56uTLRePqcazxzgulWqKD6ESnFFEicE-dFRyo0KELXGlg2rJWM9d6xoSG-nrwEpbgJT9EH7e5mzH9niAXs47ZQd_bAdKUDV0SrlsuKavopy3qxpTzCMHsLjaUmL9mTTVr_pmt7Idd7NStwe_JZ5UVON4Cj7GH-f9JZnX67Tmy2W7Eau1pv2HHX0ZprqW5uTw34sctu7xqz8wN_wNT2pMc</recordid><startdate>201608</startdate><enddate>201608</enddate><creator>Pires, C. W.</creator><creator>Botton, G.</creator><creator>Cadoná, F. C.</creator><creator>Machado, A. K.</creator><creator>Azzolin, V. F.</creator><creator>da Cruz, I. B. M.</creator><creator>Sagrillo, M. R.</creator><creator>Praetzel, J. R.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201608</creationdate><title>Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth</title><author>Pires, C. W. ; Botton, G. ; Cadoná, F. C. ; Machado, A. K. ; Azzolin, V. F. ; da Cruz, I. B. M. ; Sagrillo, M. R. ; Praetzel, J. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5032-dcd466907f4b4b66064f3d1ca43e147fbf367e27a2972c9d2247e502fd5e8ed53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>biocompatibility</topic><topic>calcium hydroxide</topic><topic>Cattle</topic><topic>deciduous tooth</topic><topic>Dentistry</topic><topic>DNA - drug effects</topic><topic>DNA Damage</topic><topic>Humans</topic><topic>Hydrocarbons, Iodinated - pharmacology</topic><topic>iodoform</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Materials Testing</topic><topic>Oxidative Stress - drug effects</topic><topic>pulpectomy</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>root canal filling materials</topic><topic>Root Canal Filling Materials - pharmacology</topic><topic>Statistics, Nonparametric</topic><topic>Tooth, Deciduous</topic><topic>Toxicity Tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pires, C. W.</creatorcontrib><creatorcontrib>Botton, G.</creatorcontrib><creatorcontrib>Cadoná, F. C.</creatorcontrib><creatorcontrib>Machado, A. K.</creatorcontrib><creatorcontrib>Azzolin, V. F.</creatorcontrib><creatorcontrib>da Cruz, I. B. M.</creatorcontrib><creatorcontrib>Sagrillo, M. R.</creatorcontrib><creatorcontrib>Praetzel, J. R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pires, C. W.</au><au>Botton, G.</au><au>Cadoná, F. C.</au><au>Machado, A. K.</au><au>Azzolin, V. F.</au><au>da Cruz, I. B. M.</au><au>Sagrillo, M. R.</au><au>Praetzel, J. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2016-08</date><risdate>2016</risdate><volume>49</volume><issue>8</issue><spage>737</spage><epage>745</epage><pages>737-745</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract><![CDATA[Aim
To evaluate the cytotoxicity, oxidative stress and genotoxicity in vitro of four iodoform pastes and three calcium hydroxide pastes.
Methodology
Peripheral blood mononuclear cells (PBMCs) and pure calf thymus DNA (dsDNA) were exposed to extracts of the pastes. Cytotoxicity was assessed with the MTT assay. Generation of reactive oxygen species (ROS) was evaluated using a DCFH‐DA assay, and lipid peroxidation was evaluated using a TBARS assay. Genotoxicity was evaluated using the alkaline comet assay and Genomodifier capacity assay (GEMO). All tests were performed after 24 h and 72 h of cell exposure, except GEMO. After performing the Kolmogorov–Smirnov test, data were analysed by Kruskal–Wallis and Dunn's post‐tests, and anova with Dunnett's post‐test, with a significance level established at P < 0.05.
Results
The MTT assay revealed that chlorhexidine, Maxitrol and neomycin sulphate + bacitracin pastes decreased cell viability after 24 h (P < 0.05). No group was associated with a significant decreased cell viability or lipid peroxidation after 72 h. Calcium hydroxide pastes increased the cell viability levels at both experimental times (P < 0.05). Lipid peroxidation was observed with the exposure of cells to calcium hydroxide pastes after 24 h (P < 0.05). Exposure to chlorhexidine, Guedes‐Pinto and calcium hydroxide pastes resulted in a significant increase in ROS after 24 h (P < 0.05), whereas iodoform pastes and Calen thickened with zinc oxide significantly increased the ROS after 72 h (P < 0.05). The comet assay revealed that exposure of the PBMCs to iodoform pastes did not damage DNA at either period of time (P > 0.05). However, chlorhexidine paste caused DNA damage in dsDNA (P < 0.05). Calcium hydroxide pastes caused DNA damage in both tests (P < 0.05).
Conclusion
The pastes varied in their ability to induce cytotoxicity, genotoxicity and oxidative stress. In general, Guedes‐Pinto, Maxitrol and neomycin sulphate + bacitracin pastes exhibited better biocompatibility in vitro.]]></abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26174479</pmid><doi>10.1111/iej.12502</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0143-2885 |
| ispartof | International endodontic journal, 2016-08, Vol.49 (8), p.737-745 |
| issn | 0143-2885 1365-2591 |
| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Analysis of Variance Animals biocompatibility calcium hydroxide Cattle deciduous tooth Dentistry DNA - drug effects DNA Damage Humans Hydrocarbons, Iodinated - pharmacology iodoform Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - metabolism Materials Testing Oxidative Stress - drug effects pulpectomy Reactive Oxygen Species - metabolism root canal filling materials Root Canal Filling Materials - pharmacology Statistics, Nonparametric Tooth, Deciduous Toxicity Tests |
| title | Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth |
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