The Influence of Selected Fingerprint Enhancement Techniques on Forensic DNA Typing of Epithelial Cells Deposited on Porous Surfaces

Fingerprints deposited at crime scene can be a source of DNA. Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfa...

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Bibliographic Details
Published in:Journal of forensic sciences 2016-01, Vol.61 (S1), p.S221-S225
Main Authors: Tsai, Li-Chin, Lee, Cheng-Chang, Chen, Chun-Chieh, Lee, James Chun-I, Wang, Sheng-Meng, Huang, Nu-En, Linacre, Adrian, Hsieh, Hsing-Mei
Format: Article
Language:English
Subjects:
DNA fingerprints
DNA typing
epithelial cells
fingerprint enhancement
forensic science
Forensic sciences
Iodine
porous surfaces
stochastic effects
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Summary:Fingerprints deposited at crime scene can be a source of DNA. Previous reports on the effects of fingerprint enhancement methods have focused mainly on fingermarks deposited in blood or saliva. Here, we evaluate the effects of fingerprint enhancement methods on fingerprints deposited on porous surfaces. We performed real‐time quantification and STR typing, the results of which indicated that two methods (iodine fuming and 1,2‐indanedione in ethyl acetate enhancement) had no effect on the quantity of DNA isolated and resultant STR alleles when compared to control samples. DNA quantities and allele numbers were lower for samples enhanced with silver nitrate and 1,2‐indanedione in acetic acid when compared to control samples. Based on DNA quantity, quality, and observable stochastic effects, our data indicated that iodine fuming and 1,2‐indanedione in ethyl acetate were the preferred options for the enhancement of fingerprints on porous surfaces.
ISSN:0022-1198
1556-4029
DOI:10.1111/1556-4029.12893