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Electrochemical magnetic beads-based immunosensing platform for the determination of α-lactalbumin in milk
[Display omitted] •First amperometric MBs-based immunosensor for α-LA with excellent sensitivity (LOD 11.0pgmL−1) and selectivity.•Determination of α-LA in different milk samples and infant formulas.•Good correlation against results provided by a commercial ELISA spectrophotometric kit.•Powerful ana...
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Published in: | Food chemistry 2016-12, Vol.213, p.595-601 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | [Display omitted]
•First amperometric MBs-based immunosensor for α-LA with excellent sensitivity (LOD 11.0pgmL−1) and selectivity.•Determination of α-LA in different milk samples and infant formulas.•Good correlation against results provided by a commercial ELISA spectrophotometric kit.•Powerful analytical tool for food safety and consumer protection.
Alpha-lactalbumin (α-LA) is one of the whey proteins in cows’ milk that has been identified as allergenic. In this work, we present, for the first time, a very sensitive magnetic beads (MBs)-based immunosensor for the determination of α-LA. A sandwich configuration involving selective capture and horseradish peroxidase-labeled detector antibodies was implemented on carboxylic acid-modified magnetic beads, captured magnetically under the surface of a disposable screen-printed carbon electrode for amperometric detection using the hydroquinone (HQ)/H2O2 system. The α-LA immunosensor exhibited a wide linear range (37.0–5000pg/ml), a low limit of detection (LOD, 11.0pg/ml) and noteworthy selectivity against other non-target proteins. The MBs-based immunosensing platform was applied successfully for the determination of α-LA in several varieties of milk (raw and UHT cows’ milk as well as human milk) and infant formulations. The results were corroborated with those obtained using a commercial ELISA method, thereby substantiating the analytical merits of this unique method. |
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ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2016.07.004 |