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Hydrogen sulfide partly mediates abscisic acid-induced heat tolerance in tobacco (Nicotiana tabacum L.) suspension cultured cells
Abscisic acid (ABA), a plant hormone, plays crucial roles in plant growth, development and response to environmental stress. Recently, hydrogen sulfide (HâS) also has emerged similar functions, but interaction between ABA and HâS in the acquisition of heat tolerance is not clear. In this study,...
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Published in: | Plant cell, tissue and organ culture tissue and organ culture, 2016-05, Vol.125 (2), p.207-214 |
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Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Abscisic acid (ABA), a plant hormone, plays crucial roles in plant growth, development and response to environmental stress. Recently, hydrogen sulfide (HâS) also has emerged similar functions, but interaction between ABA and HâS in the acquisition of heat tolerance is not clear. In this study, pretreatment of tobacco suspension cultured cells with ABA improved the survival percentage and regrowth ability, alleviated a decrease in cell vitality, increase in malondialdehyde content and electrolyte leakage of cells under heat stress, suggesting that ABA pretreatment could improve the heat tolerance of tobacco cells. In addition, treatment with ABA induced the accumulation of endogenous HâS in both tobacco cells and culture medium, increased the activity of L-cysteine desulfhydrase, a key enzyme in HâS biosynthesis. Also, ABA-induced heat tolerance was enhanced by addition of NaHS, a HâS donor, but weakened by DL-propargylglycine (specific inhibitor of HâS biosynthesis) and hypotaurine (HâS scavenger) respectively. These results suggest that ABA pretreatment could improve the heat tolerance of tobacco suspension cultured cells, and HâS, at least partly, mediated the acquisition of heat tolerance induced by ABA. |
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ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-015-0939-4 |