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Hydrogen sulfide partly mediates abscisic acid-induced heat tolerance in tobacco (Nicotiana tabacum L.) suspension cultured cells

Abscisic acid (ABA), a plant hormone, plays crucial roles in plant growth, development and response to environmental stress. Recently, hydrogen sulfide (H₂S) also has emerged similar functions, but interaction between ABA and H₂S in the acquisition of heat tolerance is not clear. In this study,...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2016-05, Vol.125 (2), p.207-214
Main Authors: Li, Zhong-Guang, Jia-Zheng Jin
Format: Article
Language:English
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Summary:Abscisic acid (ABA), a plant hormone, plays crucial roles in plant growth, development and response to environmental stress. Recently, hydrogen sulfide (H₂S) also has emerged similar functions, but interaction between ABA and H₂S in the acquisition of heat tolerance is not clear. In this study, pretreatment of tobacco suspension cultured cells with ABA improved the survival percentage and regrowth ability, alleviated a decrease in cell vitality, increase in malondialdehyde content and electrolyte leakage of cells under heat stress, suggesting that ABA pretreatment could improve the heat tolerance of tobacco cells. In addition, treatment with ABA induced the accumulation of endogenous H₂S in both tobacco cells and culture medium, increased the activity of L-cysteine desulfhydrase, a key enzyme in H₂S biosynthesis. Also, ABA-induced heat tolerance was enhanced by addition of NaHS, a H₂S donor, but weakened by DL-propargylglycine (specific inhibitor of H₂S biosynthesis) and hypotaurine (H₂S scavenger) respectively. These results suggest that ABA pretreatment could improve the heat tolerance of tobacco suspension cultured cells, and H₂S, at least partly, mediated the acquisition of heat tolerance induced by ABA.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-015-0939-4