PBT1, a novel antimicrobial protein from the biocontrol agent Bacillus subtilis XF-1 against Plasmodiophora brassicae

Plasmodiophora brassicae is a Plasmodiophorid and obligate biotrophic protist in the eukaryotic kingdom of Rhizaria. It causes clubroot disease of cruciferous crops by producing resting spores that can survive in the soil for more than ten years. Although up to date there is no effective chemical co...

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Bibliographic Details
Published in:European journal of plant pathology 2016-07, Vol.145 (3), p.583-590
Main Authors: Zhao, Jing, Wu, Yi-Xing, Ho, Hon-Hing, Chen, Zhuo-Jun, Li, Xing-Yu, He, Yue-Qiu
Format: Article
Language:English
Subjects:
Agriculture
Antimicrobial agents
Bacillus subtilis
Bacteria
Biological control
Biomedical and Life Sciences
Curvularia
E coli
Ecology
Escherichia coli
Eukaryotes
Life Sciences
Plant Pathology
Plant Sciences
Plasmodiophora brassicae
Proteins
Pseudomonas fluorescens
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Summary:Plasmodiophora brassicae is a Plasmodiophorid and obligate biotrophic protist in the eukaryotic kingdom of Rhizaria. It causes clubroot disease of cruciferous crops by producing resting spores that can survive in the soil for more than ten years. Although up to date there is no effective chemical control but now a strain of the bacterium Bacillus subtilis XF-1, isolated from China, has been found to inhibit P. brassicae and the mycelial growth of other plant-pathogenic fungi, such as Magnaporthe oryzae, Curvularia lunata,Fusarium oxysporum and so on. The antimicrobial activity of this biocontrol bacterium lies in its water-soluble protein (PBT1) which was isolated and purified by means of molecular sieve and affinity chromatography. The single band of the purified protein has a molecular mass of 25 kDa, estimated by a sodium dodecyl sulfate- polyacrylamide gel electrophoresis and it was heat resistant. The result of the sequencing proved PBT1 to be a new protein. Recombinant plasmid pQE81L-PBT1 was constructed using the coding gene of Pbt1 and plasmid PQE81-L, and expressed in Escherichia coli BL21. The fusion protein of BL21 exhibited significant detrimental effects on P. brassicae spores. In addition, pQE81L-PBT1 was successfully inserted into Pseudomonas fluorescens strain 2P24 which developed antagonistic effect to the clubroot pathogen that was not suppressed by 2P24 alone. The results showed that PBT1 protein might play a key role in the biocontrol of clubroot disease of crucifers.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-016-0905-y