Maternal–fetal transfer of indocyanine green across the perfused human placenta

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2016-07, Vol.62, p.100-105
• Main Authors: Rubinchik-Stern, Miriam, Shmuel, Miriam, Bar, Jacob, Eyal, Sara, Kovo, Michal
• Format: Article
• Language: English
• Subjects: Adult; Coloring Agents - metabolism; Cyclosporins - pharmacology; Female; Humans; Indocyanine green; Indocyanine Green - metabolism; Maternal-Fetal Exchange; Near infrared imaging; Organic anion transporting polypeptides; P-Glycoprotein; Perfusion; Placenta; Placenta - metabolism; Pregnancy; Rifampin - pharmacology
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2016.04.017

Maternal to fetal ICG transfer is decreased by organic anion transporting polypeptides (OATPs) inhibitor rifampin. [Display omitted] •The transfer of indocyanine green (ICG) across the human placenta is very low.•ICG's limited maternal-to-fetal transfer is probably mediated by placental uptake carriers of the organic anion transporting polypeptide family.•Under normal condition, the placenta is an effective barrier to ICG. Indocyanine green (ICG) is an FDA-approved near-infrared imaging probe, given also to pregnant women. We aimed to characterize ICG's transplacental transfer using the ex-vivo perfusion model. Placentas were obtained from caesarean deliveries. Cotyledons were cannulated and dually perfused. ICG, 9.6μg/mL and antipyrine (50μg/mL) were added to the maternal circulation in the absence (n=4) or the presence of the organic anion transporting polypeptide (OATPs) inhibitor rifampin (10μg/mL; n=5) or the P-glycoprotein inhibitor valspodar (2μg/mL; n=3). ICG's maternal-to-fetal transfer was evaluated over 180min. The cumulative percent of ICG in the fetal reservoir was minor. When ICG transfer was normalized to that of antipyrine, it was lower in the presence of rifampin (a 41% decrease; p