Purification and enzymatic properties of a neutral metalloprotease produced from the cold-adapted Vibrio species Pr21 isolated from deep seawater in Sagami Bay

To identify new biopolymer-degrading enzymes produced from cold-adapted microorganisms, we searched for such enzymes in bag filters used for the purification of deep seawater pumped up in Sagami Bay, Japan. We isolated a casein- and azocoll-degrading cold-adapted bacterium, and it was identified as...

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Bibliographic Details
Published in:Fisheries science 2016-07, Vol.82 (4), p.675-683
Main Authors: Ishida, Masami, Yoshida-Mishima, Chie, Maeda, Yosuke, Yamamoto, Masashi, Tsuda, Ryo, Ishii, Haruto, Urano, Naoto, Kabasawa, Hiroshi
Format: Article
Language:English
Subjects:
Analysis
Aurelia aurita
Bacteria
Biomedical and Life Sciences
Biopolymers
Brackish
Cold
Collagen
Enzymes
Filters
Fish & Wildlife Biology & Management
Fishery sciences
Food
Food Science
Freshwater & Marine Ecology
Life Sciences
Liquefaction
Marine
Microorganisms
Original Article
Physiology
Seawater
Studies
Temperature
Vibrio
Wastewater treatment
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Summary:To identify new biopolymer-degrading enzymes produced from cold-adapted microorganisms, we searched for such enzymes in bag filters used for the purification of deep seawater pumped up in Sagami Bay, Japan. We isolated a casein- and azocoll-degrading cold-adapted bacterium, and it was identified as Vibrio species Pr21. The bacterium grew between 0 and 25 °C, optimally at 20–22 °C. An extracellular 36-kDa protease, which we named PR protease, was purified from the bacterial medium. PR protease is a neutral metalloprotease that degrades azocoll (optimum temperature 40 °C). When bovine skin type I collagen (acid soluble) was used as a substrate, β chains and larger multimers decreased and α1 and α2 chains accumulated with PR protease at 10, 20, and 30 °C. This indicates that PR protease cleaves telopeptide regions of collagen molecules. PR protease accelerated the liquefaction of live moon jellyfishes Aurelia aurita at 10 and 25 °C. The present findings suggest that the bag filters used in deep seawater-pumping plants are useful for the isolation of cold-adapted microorganisms, and that PR protease has potential for applications in food processing and wastewater treatment.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-016-0993-9