Mesozooplankton grazing manipulations during in vitro iron enrichment studies in the NE subarctic Pacific

• Published in: Deep-sea research. Part II, Topical studies in oceanography Topical studies in oceanography, 1999-11, Vol.46 (11), p.2645-2668
• Main Authors: Boyd, P.W., Goldblatt, R.H., Harrison, P.J.
• Format: Article
• Language: English
• Subjects: Algae; Bacillariophyceae; Marine
• ISSN: 0967-0645; 1879-0100
• DOI: 10.1016/S0967-0645(99)00079-X

IronEx I demonstrated a rapid and marked response by grazers to Fe-induced increases in phytoplankton stocks, which was thought to be due, in part, to arrested vertical migration by mesozooplankton. These observations prompted an investigation of the relative roles of Fe enrichment and grazing pressure in controlling the magnitude of phytoplankton stocks in the NE subarctic Pacific. The grazing impact of increased mesozooplankton abundance in response to a localised Fe-induced enhancement of algal biomass was simulated by performing in vitro (6 d) grazer perturbation experiments in May 1994 and September 1995 at Ocean Station Papa (OSP), when pelagic mesozooplankton stocks are usually at their annual maximum and submaximal, respectively. Manipulations were designed to increase mesozooplankton stocks in 25L carboys after various lag-times corresponding to grazing pressure greater or equal to that in situ, and to monitor changes in chlorophyll a levels as a proxy for grazing pressure. At the onset of the experiments, in vitro mesozooplankton abundances were comparable to those in situ. Despite the addition of mesozooplankton to selected Fe-enriched carboys in May after 24, 48 and 72 h, corresponding to ca. two-fold increases in their abundances, chlorophyll a increased to ca. 2 μg l −1 in all treatments. In September, chlorophyll a levels increased five-fold to 2 μg l −1 after 4 days – but little thereafter – in the presence of up to ten-fold higher animal abundances (added at t=0) than observed in situ. Thus, Fe-induced increases in diatom growth rates were sufficiently high to escape both initial and additional grazing pressure. If and when Fe is supplied to this region, it is unlikely that mesozooplankton can respond and graze down the resulting elevated algal abundance. Theoretical calculations, based on algal growth and grazing rate data from May in this study, suggested that a greater than five-fold increase in mesozooplankton abundance, after a 48-h lag, is required to exert sufficient grazing pressure to prevent Fe-mediated increases in algal biomass. These findings are discussed in relation to the scale dependency of such events, and the pelagic ecology of other High Nitrate Low Chlorophyll regions.