Sparse serological evidence of Plasmodium vivax transmission in the Ouest and Sud-Est departments of Haiti

• Published in: Acta tropica 2016-10, Vol.162, p.27-34
• Main Authors: Weppelmann, Thomas A., von Fricken, Michael E., Lam, Brandon, Telisma, Taina, Existe, Alexandre, Lemoine, Jean F., Larkin, Joseph, Okech, Bernard A.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Antibodies, Protozoan - blood; Antibodies, Protozoan - immunology; Antigens, Protozoan - blood; Antigens, Protozoan - immunology; Cell Membrane; Child; Child, Preschool; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Haiti; Haiti - epidemiology; Humans; Immunoglobulin G - blood; Immunoglobulin G - immunology; Malaria; Malaria, Vivax - epidemiology; Malaria, Vivax - immunology; Malaria, Vivax - transmission; Male; Membrane Proteins - blood; Membrane Proteins - immunology; Merozoite Surface Protein 1 - blood; Merozoite Surface Protein 1 - immunology; Middle Aged; Plasmodium vivax; Plasmodium vivax - immunology; Plasmodium vivax - isolation & purification; Prevalence; Seroepidemiologic Studies; Seroepidemiology; Young Adult
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2016.05.011

[Display omitted] •Historical reports indicate that Plasmodium vivax malaria is likely absent in Haiti.•First seroepidemiological study of P. vivax malaria conducted in Haiti.•Large sample size and highly sensitive ELISA with two antigens (AMA-1 and MSP-1).•Found little to no evidence of sustained, low-levels of P. vivax transmission. Plasmodium vivax infections, while quite prevalent throughout South and Central America, are virtually non-existent in Haiti, where P. falciparum infections are detected in over 99% of malaria cases. Historically, few cases of P. vivax have been reported in Haiti; all of which were identified by microscopy and none were confirmed by molecular diagnostics. To further examine the transmission of P. vivax in Haiti, a cross-sectional seroepidemiological study was conducted. Whole blood was collected from 814 community members and school children ranging in age between 2 and 80 years-of-age from four locations in the Ouest and Sud-Est Departments of Haiti. After separation of serum, samples were screened for antibodies toward P. vivax apical membrane antigen (AMA-1) and merozoite surface protein-119 (MSP-1) using an indirect enzyme-linked immunosorbent assay (ELISA). Of all participants screened, 4.42% (36/814) were seropositive for AMA-1, 4.55% (37/814) were seropositive for MSP-1, 7.99% (65/814) were seropositive to either antigen, and only 0.98% (7/814) were seropositive for both antigens. Seroconversion rates (SCR) for AMA-1, MSP-1, either AMA-1 or MSP-1, and for both AMA-1 and MSP-1 estimated from the cross-sectional seroprevalence indicated rates of P. vivax transmission of less than 1% per year. Given the lack of historical evidence of P. vivax infections on the island of Hispaniola, the sparse serological evidence of antibodies toward P. vivax identified in the current study further support the notion that the transmission of P. vivax malaria might be extremely low or even completely absent in Haiti.