RNA-Seq revealed the impairment of immune defence of tilapia against the infection of Streptococcus agalactiae with simulated climate warming

• Published in: Fish & shellfish immunology 2016-08, Vol.55, p.679-689
• Main Authors: Wang, Le, Liu, Peng, Wan, Zi Yi, Huang, Shu Qing, Wen, Yan Fei, Lin, Grace, Yue, Gen Hua
• Format: Article
• Language: English
• Subjects: Animals; Climate Change; Disease; Fish Diseases - immunology; Fish Diseases - microbiology; Hot Temperature; Immune response; Oxygen metabolism; Random Allocation; RNA-Seq; Streptococcal Infections - immunology; Streptococcal Infections - microbiology; Streptococcal Infections - veterinary; Streptococcus agalactiae; Streptococcus agalactiae - physiology; Tilapia; Transcriptome; Warming
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2016.06.058

Global warming is one of the causes of disease outbreaks in fishes. Understanding its mechanisms is critical in aquaculture and fisheries. We used tilapia to study the effects of a high temperature on the infection of a bacterial pathogen Streptococcus agalactiae using RNA-Seq. We found that the dissolved oxygen level in water at 32 °C is lower than at 22 °C, and tilapia infected with the pathogen died more rapidly at 32 °C. The gene expression profiles showed significant differences in fish raised under different conditions. We identified 126 and 576 differentially expressed genes (DEGs) at 4 and 24 h post infection at 22 °C, respectively, whereas at 32 °C, the data were 312 and 1670, respectively. Almost all responding pathways at 22 °C were involved in the immune responses, whereas at 32 °C, the enriched pathways were not only involved in immune responses but also involved in oxygen and energy metabolisms. We identified significant signals of immunosuppression of immune responses at 32 °C. In addition, many of the enriched transcription factors and DEGs under positive selection were involved in immune responses, oxygen and/or energy metabolisms. Our results suggest that global warming could reduce the oxygen level in water and impair the defence of tilapia against bacterial infection. •Temperature influences mRNA profiles against bacterial infections.•Temperature influences mRNA expressions in immune and oxygen related metabolisms.•Transcription factors are involved in immune responses.•DEGs under positive selection are involved in immune responses.•High temperature impairs immune defence against bacterial infections.