Conditioned medium from human bone marrow-derived mesenchymal stem cells promotes skin moisturization and effacement of wrinkles in UVB-irradiated SKH-1 hairless mice

• Published in: Photodermatology, photoimmunology & photomedicine photoimmunology & photomedicine, 2016-05, Vol.32 (3), p.120-128
• Main Authors: Kwon, Tae-Rin, Oh, Chang Taek, Choi, Eun Ja, Kim, Soon Re, Jang, Yu-Jin, Ko, Eun Jung, Yoo, Kwang Ho, Kim, Beom Joon
• Format: Article
• Language: English
• Subjects: Administration, Cutaneous; Animals; antiwrinkle; Cell Proliferation - drug effects; Cells, Cultured; Collagen Type I - secretion; Culture Media, Conditioned - pharmacology; Elastic Tissue - drug effects; Elastic Tissue - pathology; Elastic Tissue - radiation effects; Female; Fibroblasts; Humans; Male; Matrix Metalloproteinase 1 - metabolism; Mesenchymal Stromal Cells; Mice; Mice, Hairless; MSC-CdM; photoaging; Procollagen - biosynthesis; Skin - drug effects; Skin - metabolism; Skin - radiation effects; Skin Aging - drug effects; Skin Aging - pathology; Skin Aging - radiation effects; Ultraviolet Rays - adverse effects; UVB radiation
• ISSN: 0905-4383; 1600-0781
• DOI: 10.1111/phpp.12224

Summary Background Mesenchymal stem cells (MSCs) are promising therapeutic agents for various diseases. Aims To investigate the effects of conditioned medium from human bone marrow‐derived mesenchymal stem cells (MSC‐CdM) on pro‐collagen production and wrinkle formation, we performed in vitro and in vivo experiments. Methods We assessed the effects of MSC‐CdM on proliferation and photo‐aging in human dermal fibroblasts after UVB exposure using enzyme activity assays for collagen type I secretion and MMP‐1. To determine the effect of topically applied MSC‐CdM on wrinkle formation, MSC‐CdM (1% and 10%) and vehicle (propylene glycol: ethanol, 7 : 3) were applied to the dorsal skin of UVB‐irradiated hairless mice for 8 weeks. We examined the effects on wrinkle formation by assessing visual skin grading, replica, tape stripping, transepidermal water loss (TEWL), and skin hydration measurement. We also examined histology of the lesions using hematoxylin–eosin, Masson's trichrome, and immunohistochemical staining. Results MSC‐CdM markedly reduced UV‐induced matrix metalloproteinase‐1 expression and increased pro‐collagen synthesis in a dose‐dependent manner. Our findings suggest that MSC‐CdM induces repair of dermal damage and effacement of wrinkles on UVB‐irradiated hairless mice through protective effect of hydration. Conclusion These results support an anti‐wrinkle effect of MSC‐CdM that involves increased collagen synthesis and suggest that MSC‐CdM might be a potential candidate for preventing UV‐induced skin damage.