Thermal stress induces a distinct transcriptome profile in the Pacific oyster Crassostrea gigas

Oysters are frequently subjected to heat stress during tidal emersion/immersion cycles in their habitats due to attachment on the rocky shore. To understand the effect of temperature elevation on the whole transcriptome over time, the Pacific oyster Crassostrea gigas was exposed to seawater temperat...

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Published in:Comparative biochemistry and physiology. Part D, Genomics & proteomics Genomics & proteomics, 2016-09, Vol.19, p.62-70
Main Authors: Lim, Hyun-Jeong, Kim, Bo-Mi, Hwang, In Joon, Lee, Jeong-Soo, Choi, Ik-Young, Kim, Youn-Jung, Rhee, Jae-Sung
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Animals
Computational Biology
Crassostrea - genetics
Crassostrea - growth & development
Crassostrea gigas
Gene Expression Profiling
High-Throughput Nucleotide Sequencing - methods
Molecular Sequence Annotation
Oyster
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Stress, Physiological
Temperature
Thermal stress
Transcriptome
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Summary:Oysters are frequently subjected to heat stress during tidal emersion/immersion cycles in their habitats due to attachment on the rocky shore. To understand the effect of temperature elevation on the whole transcriptome over time, the Pacific oyster Crassostrea gigas was exposed to seawater temperature 32°C for 72h from the control 20°C. RNA-seq identified differentially expressed stress responsive transcripts upon thermal stress in the gill tissues of C. gigas. The primary effect of heat stress appears to be significantly induced transcription of molecular chaperones, including members of the heat shock protein (hsp) families, while genes typically associated with protein metabolism, such as those involved in protein degradation (e.g. ATP-dependent proteolysis pathway) and biosynthesis (e.g. ribosomal protein genes), were repressed. In particular, several hsp70 isoforms and a small hsp20 maintained prolonged mRNA expressions for 72h. This study provides preliminary insights into the molecular response of C. gigas to heat stress and suggests a basis for future studies examining molecular adaptation or thermotolerance metabolism in the Pacific oyster.
ISSN:1744-117X
1878-0407
DOI:10.1016/j.cbd.2016.06.006