Effects of mutations causing hypokalaemic periodic paralysis on the skeletal muscle L-type Ca super(2+) channel expressed in Xenopus laevis oocytes

• Published in: The Journal of physiology 1999-10, Vol.520 (2), p.321-336
• Main Authors: Morrill, JA, Cannon, S C
• Format: Article
• Language: English
• Subjects: Xenopus; Xenopus laevis
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1111/j.1469-7793.1999.00321.x

1. A truncated form of the rabbit alpha sub(1S) Ca super(2+) channel subunit ( alpha sub(1S Delta C)) was expressed with the beta sub(1b), alpha sub(2) delta and gamma auxiliary subunits in Xenopus laevis oocytes. After 5-7 days, skeletal muscle L-type currents were measured (469 plus or minus 48 nA in 10 mM Ba super(2+)). All three of the auxiliary subunits were necessary to record significant L-type current. A rapidly inactivating, dihydropyridine-insensitive endogenous Ba super(2+) current was observed in oocytes expressing the auxiliary subunits without an exogenous alpha subunit. Expression of full-length alpha sub(1S) gave 10-fold smaller currents than the truncated form. 2. Three missense mutations causing hypokalaemic periodic paralysis (R528H in domain II S4 of the alpha sub(1S) subunit; R1239H and R1239G in domain IV S4) were introduced into alpha sub(1S Delta C) and expressed in oocytes. L-type current was separated from the endogenous current by nimodipine subtraction. All three of the mutations reduced L-type current amplitude ( similar to 40 % for R528H, similar to 60-70 % for R1239H and R1239G). 3. The disease mutations altered the activation properties of L-type current. R528H shifted the G(V) curve similar to 5 mV to the left and modestly reduced the voltage dependence of the activation time constant, tau sub(act). R1239H and R1239G shifted the G(V) curve similar to 5-10 mV to the right and dramatically slowed tau sub(act) at depolarized test potentials. 4. The voltage dependence of steady-state inactivation was not significantly altered by any of the disease mutations. 5. Wild-type and mutant L-type currents were also measured in the presence of (-)-Bay K8644, which boosted the amplitude similar to 5- to 7-fold. The effects of the mutations on the position of the G(V) curve and the voltage dependence of tau sub(act) were essentially the same as in the absence of agonist. Bay K-enhanced tail currents were slowed by R528H and accelerated by R1239H and R1239G. 6. We conclude that the domain IV mutations R1239H and R1239G have similar effects on the gating properties of the skeletal muscle L-type Ca super(2+) channel expressed in Xenopus oocytes, while the domain II mutation R528H has distinct effects. This result implies that the location of the substitutions is more important than their degree of conservation in determining their biophysical consequences.