Influence of different chemical pretreatments of elephant grass (Pennisetum purpureum, Schum.) used as a substrate for cellulase and xylanase production in submerged cultivation

This study evaluated the potential use of elephant grass biomass, a highly productive species, for cellulase and xylanase production by the cellulolytic mutant Penicillium echinulatum 9A02S1 in submerged cultivation, using untreated biomass, biomass pretreated with different concentrations of NaOH,...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2016-09, Vol.39 (9), p.1455-1464
Main Authors: Menegol, Daiane, Scholl, Angélica Luisi, Dillon, Aldo José Pinheiro, Camassola, Marli
Format: Article
Language:English
Subjects:
Biodiesel fuels
Biomass
Biotechnology
Cellulase
Cellulase - metabolism
Cellulose
Chemistry
Chemistry and Materials Science
Cultivation
Culture Media
Endo-1,4-beta Xylanases - metabolism
Environmental Engineering/Biotechnology
Food Science
Grasses
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Lignocellulose
Original Paper
Penicillium
Pennisetum purpureum
Poaceae - metabolism
Sodium hydroxide
Substrate Specificity
Sulfuric acid
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Summary:This study evaluated the potential use of elephant grass biomass, a highly productive species, for cellulase and xylanase production by the cellulolytic mutant Penicillium echinulatum 9A02S1 in submerged cultivation, using untreated biomass, biomass pretreated with different concentrations of NaOH, H 2 SO 4 or NH 4 OH, or biomass pretreated with H 2 O at 121 °C. For filter paper activity, all cultivation carried out with pretreated elephant grass under the evaluated conditions showed superior activity when compared with the control (untreated elephant grass). The activities of endoglucanases and β-glucosidases were higher in the cultivation prepared from pretreated samples than the control made with cellulose (Celuflok ® ). Without pretreatment, elephant grass can be used for xylanase production, enabling similar activities to those obtained in the cultivation with cellulose, reducing the enzyme production cost. These results indicate that the pretreatment of elephant grass, especially when pretreated with H 2 SO 4 , may be used as a partial or total replacement for cellulose to cellulase production, and untreated elephant grass may be used for xylanase production.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-016-1623-8