Exploring the potential reservoirs of non specific TEM beta lactamase (blaTEM) gene in the Indo-Gangetic region: A risk assessment approach to predict health hazards

Schematic representation of developed qPCR strategy in Molecular Beacon format to target blaTEM gene encoding TEM-β-lactamase resistance in Indo-Gangetic region. [Display omitted] •Prevalence of TEM-β-lactamase resistant bacteria in environmental regime.•The utility of molecular beacon based qPCR in...

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Bibliographic Details
Published in:Journal of hazardous materials 2016-08, Vol.314, p.121-128
Main Authors: Singh, Gulshan, Vajpayee, Poornima, Rani, Neetika, Amoah, Isaac Dennis, Stenström, Thor Axel, Shanker, Rishi
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Bacteria
beta-Lactam Resistance - genetics
beta-Lactamases - genetics
beta-Lactams - pharmacology
blaTEM gene
Drinking Water - microbiology
Environmental reservoirs
Genes
Geologic Sediments - microbiology
Humans
India
Microbial Sensitivity Tests
Molecular beacon
Quantitative PCR
Reservoirs
Risk
Risk Assessment
Rivers
Rivers - microbiology
Surface water
Water Microbiology
Water Pollutants
β-lactamase resistance
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Summary:Schematic representation of developed qPCR strategy in Molecular Beacon format to target blaTEM gene encoding TEM-β-lactamase resistance in Indo-Gangetic region. [Display omitted] •Prevalence of TEM-β-lactamase resistant bacteria in environmental regime.•The utility of molecular beacon based qPCR in targeting blaTEM gene.•Assay useful in formulating risk assessment strategies to combat infections.•Quantitative Microbial Risk assessment (QMRA) approach to predict human health risk. The emergence of antimicrobial resistant bacteria is an important public health and environmental contamination issue. Antimicrobials of β-lactam group accounts for approximately two thirds, by weight, of all antimicrobials administered to humans due to high clinical efficacy and low toxicity. This study explores β-lactam resistance determinant gene (blaTEM) as emerging contaminant in Indo-Gangetic region using qPCR in molecular beacon format. Quantitative Microbial Risk Assessment (QMRA) approach was adopted to predict risk to human health associated with consumption/exposure of surface water, potable water and street foods contaminated with bacteria having blaTEM gene. It was observed that surface water and sediments of the river Ganga and Gomti showed high numbers of blaTEM gene copies and varied significantly (p
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2016.04.036