Programming Surface Chemistry with Engineered Cells

We have developed synthetic gene networks that enable engineered cells to selectively program surface chemistry. E. coli were engineered to upregulate biotin synthase, and therefore biotin synthesis, upon biochemical induction. Additionally, two different functionalized surfaces were developed that...

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Bibliographic Details
Published in:ACS synthetic biology 2016-09, Vol.5 (9), p.936-941
Main Authors: Zhang, Ruihua, Heyde, Keith C, Scott, Felicia Y, Paek, Sung-Ho, Ruder, Warren C
Format: Article
Language:English
Subjects:
Biotin - metabolism
Cell Engineering - methods
Escherichia coli - genetics
Escherichia coli - metabolism
Gene Regulatory Networks - genetics
Streptavidin - metabolism
Sulfurtransferases - genetics
Synthetic Biology - methods
Tissue Engineering - methods
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Summary:We have developed synthetic gene networks that enable engineered cells to selectively program surface chemistry. E. coli were engineered to upregulate biotin synthase, and therefore biotin synthesis, upon biochemical induction. Additionally, two different functionalized surfaces were developed that utilized binding between biotin and streptavidin to regulate enzyme assembly on programmable surfaces. When combined, the interactions between engineered cells and surfaces demonstrated that synthetic biology can be used to engineer cells that selectively control and modify molecular assembly by exploiting surface chemistry. Our system is highly modular and has the potential to influence fields ranging from tissue engineering to drug development and delivery.
ISSN:2161-5063
2161-5063
DOI:10.1021/acssynbio.6b00037