Regeneration of mandibular defects using adipose tissue mesenchymal stromal cells in combination with human serum-derived scaffolds

Abstract Bone regeneration is a challenging issue. Traditional solutions bring risks, potential complications, and morbidity. The aim of the present study was to regenerate critical-sized mandible defects in athymic rats with adipose tissue mesenchymal stromal cells (AT-MSCs) in combination with hum...

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Published in:Journal of cranio-maxillo-facial surgery 2016-09, Vol.44 (9), p.1356-1365
Main Authors: Peña González, Ignacio, Álvarez-Viejo, María, Alonso-Montes, Cristina, Menéndez-Menéndez, Yolanda, Gutiérrez Álvarez, Fernando, de Vicente Rodríguez, Juan Carlos, Otero Hernández, Jesús, Meana Infiesta, Álvaro
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Animals
AT-MSCs
Autologous
Bone
Bone Regeneration - physiology
Cell Differentiation
Cells, Cultured
Dentistry
Flow Cytometry
Humans
Immunohistochemistry
Mandible - surgery
Mesenchymal Stromal Cells - cytology
Microscopy, Electron, Scanning
Models, Animal
Osteogenesis - physiology
Rats
Rats, Nude
Regeneration
Scaffold
Serum
Surgery
Tissue Scaffolds
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Summary:Abstract Bone regeneration is a challenging issue. Traditional solutions bring risks, potential complications, and morbidity. The aim of the present study was to regenerate critical-sized mandible defects in athymic rats with adipose tissue mesenchymal stromal cells (AT-MSCs) in combination with human serum-derived scaffolds. Two approaches to treatment were performed. The first approach used differentiated stromal cells that became osteogenic cell lines. The second approach used no pre-differentiation. Follow-up periods were 45 days and 90 days. Both cell types were combined with human serum-derived scaffolds. Afterward, histological (haematoxylin–eosin and Masson's Trichrome stain modified by Goldner), immunohistochemical (human vimentin and Stro-1), and radiological (microCT) studies were performed. The level of calcification between the groups was compared by analysis of variance, and statistical significance was set at p 
ISSN:1010-5182
1878-4119
DOI:10.1016/j.jcms.2016.06.012