Use of fusion protein constructs to generate potent immunotherapy and protection against scorpion toxins

• Published in: Vaccine 2001-12, Vol.20 (5), p.934-942
• Main Authors: Legros, Christian, Kaabi, Hajer, El Ayeb, Mohammed, Céard, Brigitte, Vacher, Hélène, Bougis, Pierre E, Martin-Eauclaire, Marie-France
• Format: Article
• Language: English
• Subjects: AaH I protein; AaH II protein; AaH III protein; Androctonus australis; Animals; Antibodies; Bacteriology; Base Sequence; Biological and medical sciences; Buthidae; DNA, Complementary - genetics; Epidemiology. Vaccinations; Escherichia coli; Fundamental and applied biological sciences. Psychology; General aspects; Humans; Immunization; Immunotherapy; Immunotherapy - methods; Infectious diseases; Medical sciences; Mice; Microbiology; Neuropeptides - antagonists & inhibitors; Neuropeptides - genetics; Neuropeptides - immunology; Neuropeptides - toxicity; Neurotoxins - antagonists & inhibitors; Neurotoxins - genetics; Neurotoxins - immunology; Neurotoxins - toxicity; Neutralization Tests; Plasmids - genetics; Rabbits; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Reptilian Proteins; Scorpion; Scorpion Venoms - antagonists & inhibitors; Scorpion Venoms - genetics; Scorpion Venoms - immunology; Scorpion Venoms - toxicity; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(01)00379-6

We report the use of recombinant scorpion toxins in the form of fusion proteins as antigens for immunisation in rabbits and mice: the aim was to produce in these animal models protective antisera against the most lethal α-type toxins in the venom from the North African scorpion Androctonus australis. The cDNAs encoding AaH I, AaH II and AaH III (the three major α-type toxins acting on voltage-sensitive sodium channels) were fused to the sequence encoding the maltose binding protein (MBP). The constructs (MBP- AaH I, MBP- AaH II, MBP- AaH I+II and MBP- AaH III) were expressed in Escherichia coli, and resulting fusion proteins were translocated to the periplasmic space. The recombinant fusion proteins were characterised and used as antigens to generate antibodies in rabbits. These antibodies raised specifically recognised their corresponding radiolabelled-toxin with affinities in the 0.1 nM range. In vitro neutralisation assays indicated that 1 ml of serum raised against a mixture of fusion proteins was able to neutralise 15 LD 50 of the toxic fraction ( AaH-G50) purified from the crude venom by molecular filtration through Sephadex G50. In vivo, the fusion proteins induced a long-term protection in mice against the lethal effects of AaH-G50 or of the native toxins. Ten weeks after the beginning of the immunisation programme, mice were challenged with various toxins or AaH-G50 doses. Mice were fully protected against three LD 50 of AaH-G50. Our work shows that fusion protein constructs can be used as a vaccine providing efficient immune protection against A. australis venom.