Bevacizumab anticancer monoclonal antibody: native and denatured redox behaviour

[Display omitted] •Electrochemical oxidation of native and denatured BEVA was investigated.•Native BEVA, due to tyrosine, first tryptophan and cysteine, one amino acid residues oxidation peak.•BEVA-thin film on GCE was essentially capacitive.•Denatured BEVA-thin film on GCE showed also second trypto...

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Bibliographic Details
Published in:Electrochimica acta 2016-07, Vol.206, p.246-253
Main Authors: Issaad, Fatima Z., Tomé, Luciana I.N., Marques, Nuno V., Mouats, Chabane, Diculescu, Victor C., Oliveira-Brett, Ana Maria
Format: Article
Language:English
Subjects:
antibody
bevacizumab
denaturing agents
Electrochemical impedance spectroscopy
Electrochemical oxidation
Glassy carbon
glassy carbon electrode
Monoclonal antibodies
Oxidation
Residues
Surface chemistry
Voltammetry
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Summary:[Display omitted] •Electrochemical oxidation of native and denatured BEVA was investigated.•Native BEVA, due to tyrosine, first tryptophan and cysteine, one amino acid residues oxidation peak.•BEVA-thin film on GCE was essentially capacitive.•Denatured BEVA-thin film on GCE showed also second tryptophan and cysteine residues oxidation peaks. Bevacizumab (BEVA) is a monoclonal antibody (mAb) used in clinical oncology to treat certain types of metastatic cancers. The electrochemical oxidation of native and denatured BEVA was investigated in solution over a wide pH range and using BEVA-thin film voltammetry on a glassy carbon electrode. For native BEVA, only one pH-dependent oxidation peak, corresponding to tyrosine and tryptophan amino acid residues oxidation, was observed. The interfacial behaviour and adsorption of BEVA at the glassy carbon surface were evaluated by voltammetry and electrochemical impedance spectroscopy. The unfolding of the protein 3D morphological structure occurred upon denaturation with chemical agents, denaturing agent sodium dodecyl sulphate, and the reductants tris(2-carboxyethyl)phosphine and dithiothreitol, was electrochemically characterized. The electrochemical oxidation of denatured BEVA-thin film on GCE showed additional cysteine and histidine residues oxidation peaks.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2016.04.097