Increasing the catalytic activity of Bilirubin oxidase from Bacillus pumilus: Importance of host strain and chaperones proteins

• Published in: Journal of biotechnology 2016-07, Vol.230, p.19-25
• Main Authors: Gounel, Sébastien, Rouhana, Jad, Stines-Chaumeil, Claire, Cadet, Marine, Mano, Nicolas
• Format: Article
• Language: English
• Subjects: Bacillus; Bacillus pumilus; Bacillus pumilus - enzymology; Bacillus pumilus - genetics; Bacteria; Bacterial laccase; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bilirubin oxidase; Chaperone proteins; Copper; Disulfides; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Heterologous expression; Molecular Chaperones - metabolism; Oxidase; Oxidoreductases Acting on CH-CH Group Donors - chemistry; Oxidoreductases Acting on CH-CH Group Donors - genetics; Oxidoreductases Acting on CH-CH Group Donors - metabolism; Proteins; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Strain; Strategy
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2016.04.035

•We investigated the role of folding chaperone proteins on the expression of MCOs in E. coli.•It leads to an increased amount of bacterial recombinant MCO by a factor 15.•Changing the strain of production increases the catalytic rate constant of BOD from B. pumilus.•Electrochemical properties of the immobilized BOD were improved by 70%. Aggregation of recombinant proteins into inclusion bodies (IBs) is the main problem of the expression of multicopper oxidase in Escherichia coli. It is usually attributed to inefficient folding of proteins due to the lack of copper and/or unavailability of chaperone proteins. The general strategies reported to overcome this issue have been focused on increasing the intracellular copper concentration. Here we report a complementary method to optimize the expression in E. coli of a promising Bilirubin oxidase (BOD) isolated from Bacillus pumilus. First, as this BOD has a disulfide bridge, we switched E.coli strain from BL21 (DE3) to Origami B (DE3), known to promote the formation of disulfide bridges in the bacterial cytoplasm. In a second step, we investigate the effect of co-expression of chaperone proteins on the protein production and specific activity. Our strategy allowed increasing the final amount of enzyme by 858% and its catalytic rate constant by 83%.