Extracellular matrix-based cryogels for cartilage tissue engineering

[Display omitted] In this study, we investigated various highly porous extracellular matrix (ECM)-based cryogels for cartilage tissue engineering. For the fabrication of ECM-based cryogels, either methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) were cross-linked alon...

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Bibliographic Details
Published in:International journal of biological macromolecules 2016-12, Vol.93 (Pt B), p.1410-1419
Main Authors: Han, Min-Eui, Kim, Su-Hwan, Kim, Hwan D., Yim, Hyun-Gu, Bencherif, Sidi A., Kim, Tae-Il, Hwang, Nathaniel S.
Format: Article
Language:English
Subjects:
Animals
Cartilage tissue engineering
Cartilage, Articular - cytology
Cartilage, Articular - physiology
Cell Survival
Cells, Cultured
Chondrocytes - physiology
Chondroitin sulfate
Cryogel
Cryogels - chemistry
Extracellular Matrix - chemistry
Female
Hyaluronic acid
Implants, Experimental
Mice, Inbred BALB C
Mice, Nude
Poly (ethylene glycol) diacrylates
Porosity
Regeneration
Regenerative Medicine
Tissue Engineering
Tissue Scaffolds - chemistry
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Summary:[Display omitted] In this study, we investigated various highly porous extracellular matrix (ECM)-based cryogels for cartilage tissue engineering. For the fabrication of ECM-based cryogels, either methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) were cross-linked along with poly (ethylene glycol) diacrylates (PEGDA) via free radical polymerization under freezing conditions. This procedure induces ice crystallization (used as a porogen) prior polymer crosslinking in which, after complete cryopolymerization, a thawing process transforms the ice crystals into a unique interconnected macroporous structure within ECM-cryogels. The developed ECM-cryogels exhibited an average macroporosity of 75% and supported the infiltration of chondrocyteds. When rabbit chondrocytes were cultured on ECM-cryogels, MeCS-based cryogels stimulated aggrecan gene expression and GAG accumulation, whereas MeHA-based cryogels stimulated type II collagen gene expression and collagen accumulation. These results demonstrate that design of ECM-based cryogels can play an important role in promoting specific ECM proteins secretion for cartilage tissue engineering.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2016.05.024