New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography

Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same sample. Rare dynamic events or specific objects can be identified, targeted and imaged by electron microscopy or tomography. To combine it with st...

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Bibliographic Details
Published in:Journal of structural biology 2017-02, Vol.197 (2), p.83-93
Main Authors: Schorb, Martin, Gaechter, Leander, Avinoam, Ori, Sieckmann, Frank, Clarke, Mairi, Bebeacua, Cecilia, Bykov, Yury S., Sonnen, Andreas F.-P., Lihl, Reinhard, Briggs, John A.G.
Format: Article
Language:English
Subjects:
Correlative light and electron microscopy
Cryo-electron microscopy
Cryo-electron tomography
Cryo-fluorescence microscopy
Cryoelectron Microscopy - instrumentation
Cryoelectron Microscopy - methods
Electron Microscope Tomography - instrumentation
Electron Microscope Tomography - methods
Microscopy - instrumentation
Microscopy - methods
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Software
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