Bacterial selection by mycospheres of Atlantic Rainforest mushrooms

• Published in: Antonie van Leeuwenhoek 2016-10, Vol.109 (10), p.1353-1365
• Main Authors: Halsey, Joshua Andrew, de Cássia Pereira e Silva, Michele, Andreote, Fernando Dini
• Format: Article
• Language: English
• Subjects: Agaricales - classification; Agaricales - genetics; Agaricales - physiology; Bacteria; Bacterial Physiological Phenomena; Biomedical and Life Sciences; Brazil; Denaturing Gradient Gel Electrophoresis; DNA, Fungal; Life Sciences; Medical Microbiology; Microbiology; Mushrooms; Original Paper; Plant Sciences; Pseudomonadaceae; Rainforest; Rainforests; Soil Microbiology; Soil Science & Conservation; Studies
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-016-0734-1

This study focuses on the selection exerted on bacterial communities in the mycospheres of mushrooms collected in the Brazilian Atlantic Rainforest. A total of 24 paired samples (bulk soil vs. mycosphere) were assessed to investigate potential interactions between fungi and bacteria present in fungal mycospheres. Prevalent fungal families were identified as Marasmiaceae and Lepiotaceae (both Basidiomycota ) based on ITS partial sequencing. We used culture-independent techniques to analyze bacterial DNA from soil and mycosphere samples. Bacterial communities in the samples were distinguished based on overall bacterial, alphaproteobacterial, and betaproteobacterial PCR-DGGE patterns, which were different in fungi belonging to different taxa. These results were confirmed by pyrosequencing the V4 region of the 16S rRNA gene (based on five bulk soil vs. mycosphere pairs), which revealed the most responsive bacterial families in the different conditions generated beneath the mushrooms, identified as Bradyrhizobiaceae , Burkholderiaceae , and Pseudomonadaceae . The bacterial families Acetobacteraceae , Chrhoniobacteraceae , Planctomycetaceae , Conexibacteraceae , and Burkholderiaceae were found in all mycosphere samples, composing the core mycosphere microbiome. Similarly, some bacterial groups identified as Koribacteriaceae, Acidobacteria ( Solibacteriaceae ) and an unclassified group of Acidobacteria were preferentially present in the bulk soil samples (found in all of them). In this study we depict the mycosphere effect exerted by mushrooms inhabiting the Brazilian Atlantic Rainforest, and identify the bacteria with highest response to such a specific niche, possibly indicating the role bacteria play in mushroom development and dissemination within this yet-unexplored environment.