New Microsatellite Markers for Two Sympatric Tinamou Species, the Ornate Tinamou (Nothoprocta ornata) and Darwin's Nothura (Nothura darwinii)

Tinamous (Tinamidae) represent one of the most ancient living avian lineages but their life history traits are relatively unstudied. Here we identified microsatellite loci for two sympatric tinamou species, the Ornate Tinamou (Nothoprocta ornata) and the Darwin's Nothura (Nothura darwinii) from...

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Bibliographic Details
Published in:Avian biology research 2016-09, Vol.9 (3), p.139-146
Main Authors: Deck, Lina Maria Giraldo, Habel, Jan Christian, Curto, Manuel, Husemann, Martin, Sturm, Sarah, Garitano-Zavala, Álvaro, Meimberg, Harald
Format: Article
Language:English
Subjects:
Alleles
DNA sequencing
Genetic markers
Heterozygosity
Life history
Microsatellites
Nothoprocta ornata
Nothura
Nothura darwinii
Population genetics
Population structure
Social behavior
Species
Sympatric populations
Tinamidae
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Summary:Tinamous (Tinamidae) represent one of the most ancient living avian lineages but their life history traits are relatively unstudied. Here we identified microsatellite loci for two sympatric tinamou species, the Ornate Tinamou (Nothoprocta ornata) and the Darwin's Nothura (Nothura darwinii) from low coverage Illumina sequencing of genomic DNA. The experiment yielded a large number of candidate loci. We designed primers and tested them for successful amplification in 1 to 2 populations of the target species, tested for deviation from the Hardy–Weinberg equilibrium and the presence of null alleles, the levels of polymorphism and potential cross-amplification. All 30 and 24 loci amplified consistently, in the Ornate Tinamou and in Darwin's Nothura, respectively. In the Ornate Tinamou, 25 loci were polymorphic and in the Darwin's Nothura 12, with 2 to 14 alleles per locus in both species. The expected heterozygosity ranged from 0.054 to 0.917 in the Ornate Tinamou and from 0.044 to 0.908 in the Darwin's Nothura. 23 (40%) of 54 loci were successfully cross-amplified. These newly discovered, polymorphic microsatellite loci represent a valuable tool for future studies on social behaviour, parentage and genetic population structure in tinamous.
ISSN:1758-1559
1758-1567
DOI:10.3184/175815515X14503747783157