Back-trajectory modelling and DNA-based species-specific detection methods allow tracking of fungal spore transport in air masses

• Published in: The Science of the total environment 2016-11, Vol.571, p.658-669
• Main Authors: Grinn-Gofroń, Agnieszka, Sadyś, Magdalena, Kaczmarek, Joanna, Bednarz, Aleksandra, Pawłowska, Sylwia, Jedryczka, Malgorzata
• Format: Article
• Language: English
• Subjects: Air Microbiology; Ascomycota - genetics; Ascomycota - isolation & purification; Brassica napus; Brassica napus - microbiology; DNA, Fungal - analysis; DNA-based detection method; Environmental Monitoring - methods; HYSPLIT; Leptosphaeria; Leptosphaeria biglobosa; Leptosphaeria maculans; Long distance transport; Models, Theoretical; Oilseed rape; Plant Diseases - microbiology; Poland; Real-time PCR; Real-Time Polymerase Chain Reaction - methods; Species Specificity; Spores, Fungal - isolation & purification
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2016.07.034

Recent advances in molecular detection of living organisms facilitate the introduction of novel methods to studies of the transport of fungal spores over large distances. Monitoring the migration of airborne fungi using microscope based spore identification is limited when different species produce very similar spores. In our study, DNA-based monitoring with the use of species-specific probes allowed us to track the aerial movements of two important fungal pathogens of oilseed rape (Brassica napus L.), i.e., Leptosphaeria maculans and Leptosphaeria biglobosa, which have identical spore shape and size. The fungi were identified using dual-labelled fluorescent probes that were targeted to a β-tubulin gene fragment of either Leptosphaeria species. Spore identification by Real-Time PCR techniques capable of detecting minute amounts of DNA of selected fungal species was combined with back-trajectory analysis, allowing the tracking of past movements of air masses using the Hybrid Single Particle Lagrangian Integrated Trajectory model. Over a study period spanning the previous decade (2006–2015) we investigated two specific events relating to the long distance transport of Leptosphaeria spp. spores to Szczecin in North-West Poland. Based on the above mentioned methods and the results obtained with the additional spore sampler located in nearby Szczecin, and operating at the ground level in an oilseed rape field, we have demonstrated that on both occasions the L. biglobosa spores originated from the Jutland Peninsula. This is the first successful attempt to combine analysis of back-trajectories of air masses with DNA-based identification of economically important pathogens of oilseed rape in Europe. In our studies, the timing of L. biglobosa ascospore dispersal in the air was unlikely to result in the infection of winter oilseed rape grown as a crop plant. However, the fungus could infect other host plants, such as vegetable brassicas, cruciferous weeds, spring rapeseed and winter rapeseed growing as a volunteer plant. [Display omitted] •We examined sudden and very high rises in Leptosphaeria spp. spore concentration•Molecular methods identified the species as Leptosphaeria biglobosa•We have ruled out local sources of L. biglobosa ascospores for these spikes•We used a Lagrangian model to trace back the origin of the fungus•Back-trajectories combined with DNA-based identification is a new powerful method