Genomic and functional features of the biosurfactant producing Bacillus sp. AM13

Genomic studies provide deeper insights into secondary metabolites produced by diverse bacterial communities, residing in various environmental niches. This study aims to understand the potential of a biosurfactant producing Bacillus sp. AM13, isolated from soil. An integrated approach of genomic an...

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Bibliographic Details
Published in:Functional & integrative genomics 2016-09, Vol.16 (5), p.557-566
Main Authors: Shaligram, Shraddha, Kumbhare, Shreyas V., Dhotre, Dhiraj P., Muddeshwar, Manohar G., Kapley, Atya, Joseph, Neetha, Purohit, Hemant P., Shouche, Yogesh S., Pawar, Shrikant P.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Bacillus
Bacillus - genetics
Bacillus - metabolism
Bacteria
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biochemistry
Bioinformatics
Biomedical and Life Sciences
Cell Biology
Genome, Bacterial
Genomes
Genomics
Life Sciences
Microbial Genetics and Genomics
Original Article
Peptide Synthases - genetics
Peptide Synthases - isolation & purification
Peptides
Plant Genetics and Genomics
Secondary Metabolism - genetics
Surface-Active Agents - metabolism
Surfactants
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Summary:Genomic studies provide deeper insights into secondary metabolites produced by diverse bacterial communities, residing in various environmental niches. This study aims to understand the potential of a biosurfactant producing Bacillus sp. AM13, isolated from soil. An integrated approach of genomic and chemical analysis was employed to characterize the antibacterial lipopeptide produced by the strain AM13. Genome analysis revealed that strain AM13 harbors a nonribosomal peptide synthetase (NRPS) cluster; highly similar with known biosynthetic gene clusters from surfactin family: lichenysin (85 %) and surfactin (78 %). These findings were substantiated with supplementary experiments of oil displacement assay and surface tension measurements, confirming the biosurfactant production. Further investigation using LCMS approach exhibited similarity of the biomolecule with biosurfactants of the surfactin family. Our consolidated effort of functional genomics provided chemical as well as genetic leads for understanding the biochemical characteristics of the bioactive compound.
ISSN:1438-793X
1438-7948
DOI:10.1007/s10142-016-0506-z