Photo-induced cytotoxicity of malonic acid [C 60]fullerene derivatives and its mechanism

• Published in: Toxicology in vitro 2002-02, Vol.16 (1), p.41-46
• Main Authors: Yang, X.L., Fan, C.H., Zhu, H.S.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Carbon - chemistry; Carbon - toxicity; Cell Cycle; Cell Division - drug effects; Cell Division - radiation effects; Cell growth; Cell Survival - drug effects; Cell Survival - radiation effects; Chemical and industrial products toxicology. Toxic occupational diseases; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Flow Cytometry; Fullerenes; HeLa Cells - drug effects; HeLa Cells - radiation effects; Humans; Light; Malonates - chemistry; Malonates - toxicity; malonic acid; Malonic acid C 60 derivatives; Mannitol - pharmacology; Medical sciences; Photo-induced; Reactive oxygen species; Structure-Activity Relationship; Tetrazolium Salts - metabolism; Thiazoles - metabolism; Toxicology; Various organic compounds
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/S0887-2333(01)00102-3

The biological activities of fullerenes have attracted extensive attention in recent years. The aim of this paper is to study the relation of the photo-induced cytotoxicity of fullerene derivatives to their chemical structures as well as the possible cellular mechanism involved in the photocytotoxicity. Three C 60 derivatives with two to four malonic acid groups (DMA C 60, TMA C 60 and QMA C 60) were prepared and the cytotoxicity of these compounds against HeLa cells was determined by MTT. Cell cycle was measured by flow cytometry. The results showed that the cytotoxicity of the malonic acid C 60 derivatives was irradiation- and dose-dependent. The sequence of their photo-induced growth inhibition was DMA C 60>TMA C 60>QMA C 60. Hydroxyl radical quencher mannitol (10 m m) was not able to prevent cells from the damage induced by irradiated DMA C 60. DMA C 60, together with irradiation, was found to have an ability of inducing a decrease in the number of G 1 cells from 63 to 42% and a rise in that of G 2+M cells from 6 to 26%. These data indicated that the number of malonic acid molecules added to C 60 played an important role in the phototoxicity, and the blockage of cell cycle might be a mechanism of this activity.