Genetic map locations for orthologous Vp1 genes in wheat and rice

• Published in: Theoretical and applied genetics 1999-02, Vol.98 (2), p.281-284
• Main Authors: Bailey, P.C, McKibbin, R.S, Lenton, J.R, Holdsworth, M.J, Flintham, J.E, Gale, M.D
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; chromosome mapping; Chromosomes; Classical and quantitative genetics; Classical and quantitative genetics. Population genetics. Molecular genetics; Classical genetics, quantitative genetics, hybrids; Cloning; color; Embryos; Fundamental and applied biological sciences. Psychology; gene location; Generalities. Genetics. Plant material; Genes; Genetic aspects; genetic markers; Genetic transcription; Genetics and breeding of economic plants; Genetics of eukaryotes. Biological and molecular evolution; genome; Genomes; Grain; Harvest; linkage (genetics); Oryza sativa; Physiological aspects; Plant genetics; Pteridophyta, spermatophyta; r locus; regulator genes; Research parks; restriction fragment length polymorphism; Rice; seed dormancy; Seeds; testa; Transcription factors; Triticum aestivum; Vegetals; VIVIPAROUS-1; Vp1 gene; vp1 locus; Wheat
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051069

Chromosome locations for gene orthologues of the dormancy-related maize transcription factor VIVIPAROUS-1, encoded by the Vp1 locus on maize chromosome 3, were determined in wheat (Triticum aestivum L.) and rice (Oryza sativa L.) via linkage to markers on existing molecular maps using a cDNA of a wheat Vp1 orthologue as a probe in genomic Southern analyses. Vp1-orthologous loci were detected on the long arms of wheat chromosomes 3A, 3B and 3D [Xlars 10 (taVp1) loci] and rice chromosome 1 (osVp1), in line with previous evidence of synteny between these regions of the rice and wheat genomes and chromosome 3 of maize. The wheat loci mapped some 30 cM from the centromeres and some 30 cM proximal to the red grain (R) loci that control seed colour and coat-imposed dormancy. This unequivocal, genetic separation of the Vp1 and R loci may offer an opportunity for improving resistance to pre-harvest sprouting in wheat by combining the coat-imposed dormancy associated with red seed colour and true embryo dormancy regulated by Vp1.