In vitro and ex vivo strategies for intracellular delivery

Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety o...

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Bibliographic Details
Published in:Nature (London) 2016-10, Vol.538 (7624), p.183-192
Main Authors: Stewart, Martin P., Sharei, Armon, Ding, Xiaoyun, Sahay, Gaurav, Langer, Robert, Jensen, Klavs F.
Format: Article
Language:English
Subjects:
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13/62
631/80
639/925
Acids
Animals
Cell Membrane - metabolism
Cellular biology
Clinical trials
DNA sequencing
Drug Delivery Systems - methods
Gene therapy
Genetic engineering
Humanities and Social Sciences
Humans
In Vitro Techniques
Intracellular Space - genetics
Intracellular Space - metabolism
Lab-On-A-Chip Devices
Medical research
Methods
Microfluidics - methods
multidisciplinary
Nanomaterials
Nanotechnology
Nanotechnology - methods
Proteins
review-article
Science
Stem cells
Transcription factors
Transfection - methods
Vectors (Biology)
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Summary:Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field. Intracellular delivery methods, which are critical to both fundamental research applications and cell-based therapies, are reviewed, concentrating on membrane-disruption-based methods and the use of nanotechnology, microfluidics and laboratory-on-chip technology. Latest developments in intracellular delivery Intracellular delivery to mammalian cells has become critical to both fundamental research applications and cell-based therapies. In this Review, Klavs Jensen and colleagues discuss recent work on developing in vitro and ex vivo strategies for cytosolic delivery of macromolecules, concentrating on membrane-disruption-based methods and the use of nanotechnology, microfluidics and laboratory-on-a-chip technology.
ISSN:0028-0836
1476-4687
DOI:10.1038/nature19764