Development and validation of ultrafast LC–MS/MS method for quantification of anti-influenza agent camphecene in whole rat blood using dried blood spots and its application to pharmacokinetic studies

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2016-11, Vol.1036-1037, p.136-141
• Main Authors: Rogachev, Artem D., Yarovaya, Olga I., Ankov, Sergey V., Khvostov, Mikhail V., Tolstikova, Tatyana G., Pokrovsky, Andrey G., Salakhutdinov, Nariman F.
• Format: Article
• Language: English
• Subjects: Animals; Anti-influenza; Antiviral Agents - blood; Camphecene; Camphor - analogs & derivatives; Camphor - blood; Chromatography, High Pressure Liquid - methods; Dried Blood Spot Testing - economics; Dried Blood Spot Testing - methods; Dried blood spots; Ethanolamines - blood; Humans; Influenza, Human - blood; Influenza, Human - drug therapy; LC–MS/MS; Limit of Detection; Male; Orthomyxoviridae Infections - blood; Orthomyxoviridae Infections - drug therapy; Rats; Rats, Wistar; Tandem Mass Spectrometry - economics; Tandem Mass Spectrometry - methods; Time Factors
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2016.10.009

•The dried blood spot (DBS) technique was used for sample preparation.•Twenty microlitres of whole blood from animal are enough for analysis.•Less than 2min was needed for each analytical run.•The method was validated according to the FDA and EMA guidelines for bioanalytical methods.•The method suitability was demonstrated by a pharmacokinetic study in rats. A fast, selective and sensitive procedure for quantitation of the camphor-based anti-influenza agent camphecene in whole rat blood was developed and validated using dried blood spots and LC–MS/MS. The method was validated according to recommendations of the FDA and EMA in terms of selectivity, linearity, accuracy, precision, recovery, matrix factor, stability, and carry-over. Sample preparation included spotting 20μL of whole blood taken from the tail vein onto the paper, drying and extracting the analyte, followed by evaporation of the solvent and analysis of the residue. HPLC separations were run on a reversed-phase microcolumn; the time of analysis was less than 2min. MS/MS detection was performed on a triple quadrupole mass-spectrometer using multiple reaction monitoring (MRM) mode. Transitions 196.4→122.2/153.3 and 152.2→93.1/107.2 were monitored for camphecene and 2-adamantylamine hydrochloride (internal standard), respectively. The intra- and inter-day precisions and accuracies, matrix factor, carry-over and recovery were within acceptable limits. Despite low extraction recovery (less than 2%), the sensitivity of the method was enough to detect the analyte in the concentration range 50–2500ng/mL. The application of the method was shown in pharmacokinetic studies of camphecene in rats at a dose of 10mg/kg.