Reactive oxygen species depolymerize hyaluronan: involvement of the hydroxyl radical

We have previously demonstrated that reactive oxygen species (ROS) are involved in cartilage degradation. A decrease in the size of hyaluronan (HA), which is the major macromolecule in synovial fluid and is responsible for imparting viscosity to it, is reported in arthritis patients. The purpose of...

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Bibliographic Details
Published in:Pathophysiology (Amsterdam) 2003-09, Vol.9 (4), p.215-220
Main Authors: Yamazaki, Kenji, Fukuda, Kanji, Matsukawa, Masataka, Hara, Fumihiko, Yoshida, Koji, Akagi, Masao, Munakata, Hiroshi, Hamanishi, Chiaki
Format: Article
Language:English
Subjects:
Arthritis
Cartilage
Degradation
Hyaluronan
Hydroxyl radical
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Summary:We have previously demonstrated that reactive oxygen species (ROS) are involved in cartilage degradation. A decrease in the size of hyaluronan (HA), which is the major macromolecule in synovial fluid and is responsible for imparting viscosity to it, is reported in arthritis patients. The purpose of this study is to determine the ROS that depolymerize HA. The luminol derivative, L-012, was used to determine the generation of ROS. To generate hydroxyl radicals, a mixture of hydrogen peroxide (H 2O 2) and ferrous ions (Fe 2+) was added to HA. The antioxidants and the depolymerization of HA were studied in this system. The hydroxyl radical is one of the ROS, causing the depolymerization of HA, which reacts with L-01. These data suggest that hydroxyl radicals play an important role at the site of inflammation.
ISSN:0928-4680
1873-149X
DOI:10.1016/S0928-4680(03)00024-5