Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

In this study, the effect of different structures (flat, expanded, and electrospun) of polytetrafluoroethylene (PTFE) on the interactions of endothelial cells (ECs), smooth muscle cells (SMCs), and platelets was investigated. In addition, the mechanisms that govern the interactions between ECs, SMCs...

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Published in:Journal of biomedical materials research. Part A 2016-09, Vol.104 (9), p.2291-2304
Main Authors: Lamichhane, Sujan, Anderson, Jordan A., Remund, Tyler, Sun, Hongli, Larson, Mark K., Kelly, Patrick, Mani, Gopinath
Format: Article
Language:English
Subjects:
biomaterials surface
Blood Platelets - cytology
Blood Platelets - metabolism
cardiovascular
Cell Line
Electrospinning
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - metabolism
Humans
Materials Testing
Muscles
Myocytes, Smooth Muscle - cytology
Myocytes, Smooth Muscle - metabolism
Platelet Adhesiveness
Platelets
polytetrafluoroethylene
Polytetrafluoroethylene - chemistry
Polytetrafluoroethylenes
smooth muscle cells
Surface chemistry
Surface Properties
Topography
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Summary:In this study, the effect of different structures (flat, expanded, and electrospun) of polytetrafluoroethylene (PTFE) on the interactions of endothelial cells (ECs), smooth muscle cells (SMCs), and platelets was investigated. In addition, the mechanisms that govern the interactions between ECs, SMCs, and platelets with different structures of PTFE were discussed. The surface characterizations showed that the different structures of PTFE have the same surface chemistry, similar surface wettability and zeta potential, but uniquely different surface topography. The viability, proliferation, morphology, and phenotype of ECs and SMCs interacted with different structures of PTFE were investigated. Expanded PTFE (ePTFE) provided a relatively better surface for the growth of ECs. In case of SMC interactions, although all the different structures of PTFE inhibited SMC growth, a maximum inhibitory effect was observed for ePTFE. In case of platelet interactions, the electrospun PTFE provided a better surface for preventing the adhesion and activation of platelets. Thus, this study demonstrated that the responses of ECs, SMCs, and platelets strongly dependent on the surface topography of the PTFE. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2291–2304, 2016.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.35763