When the Label Matters: Adsorption of Labeled and Unlabeled Proteins on Charged Surfaces

Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can si...

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Bibliographic Details
Published in:Nano letters 2015-11, Vol.15 (11), p.7508-7513
Main Authors: Romanowska, Julia, Kokh, Daria B, Wade, Rebecca C
Format: Article
Language:English
Subjects:
Adsorption
Animals
Chickens
Fluorescence
Fluorescent Dyes - chemistry
Labels
Models, Molecular
Molecular Docking Simulation
Molecular structure
Monitors
Muramidase - chemistry
Nanostructure
Proteins
Staining and Labeling
Surface chemistry
Surface Properties
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Summary:Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can significantly change the interaction properties of a protein. Thus, the results of experiments with fluorescently labeled proteins should be interpreted by modeling the structures and computing the interaction properties of both labeled and unlabeled species.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.5b03168