Glutamate microbiosensors based on Prussian Blue modified carbon fiber electrodes for neuroscience applications: In-vitro characterization

[Display omitted] •A novel Prussian Blue-modified microbiosensor for neuroscience applications is reported.•Prussian Blue allows the detection of enzyme-generated hydrogen peroxide at a low applied potential.•The most important steps during biosensor assembly have been carefully revised.•Its low dim...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2016-11, Vol.235, p.117-125
Main Authors: Salazar, P., Martín, M., O’Neill, R.D., González-Mora, J.L.
Format: Article
Language:English
Subjects:
Biosensor
Biosensors
Carbon fiber electrode
Carbon fibers
Electrodes
Glutamate
Glutamates
Interference
Mathematical analysis
Monitoring
Neurochemistry
Platinum
Poly-o-phenylenediamine
Prussian blue
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Summary:[Display omitted] •A novel Prussian Blue-modified microbiosensor for neuroscience applications is reported.•Prussian Blue allows the detection of enzyme-generated hydrogen peroxide at a low applied potential.•The most important steps during biosensor assembly have been carefully revised.•Its low dimensions and excellent sensitivity and selectivity are adequate for neurochemical monitoring of brain glutamate. Herein we report a Prussian Blue modified carbon fiber electrode (CFE/PB) to be used in microbiosensors for glutamate monitoring in physiological applications as an alternative to the classical Pt and Pt-Ir transducers. Their low dimensions (∼250μm CFE length and ∼10μm diameter) are advantageous for measuring in living tissues. In addition, PB-modified microelectrodes allow the detection of enzyme-generated hydrogen peroxide at a low applied potential (∼0.0V against SCE), contrasting the high potential used in many previous designs (∼0.7V), decreasing the endogenous interference contributions. Moreover, the electrosynthesized polymer, poly-o-phenylenediamine (PoPD), was used to improve biosensor stability and selectivity. CFE/PB was conveniently characterized using impedance, Raman and XPS spectroscopies. Optimization of the fabrication procedure and analytical conditions is described, including activation of CFE/PB, enzyme enrichment, cross-linking, stabilization and anti-interference. A range of analytical parameters were also characterized such as sensitivity, limit of detection, linear range, and enzymatic loading. Finally, an optimized biosensor displaying a linear sensitivity of 135±2nAμM−1cm−2 (n=3), LOD of
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.05.057