Detergent assisted lipid extraction from wet yeast biomass for biodiesel: A response surface methodology approach

•Wet oleaginous yeast biomass was used for lipid extraction.•Biodegradable anionic detergent (N-lauroyl sarcosine) was used for cell disruption.•N-lauroyl sarcosine minimized lipid extraction time.•N-lauroyl sarcosine had no effect on fatty acid profiles. The lipid extraction from the microbial biom...

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Bibliographic Details
Published in:Bioresource technology 2016-10, Vol.218, p.667-673
Main Authors: Yellapu, Sravan Kumar, Bezawada, Jyothi, Kaur, Rajwinder, Kuttiraja, Mathiazhakan, Tyagi, Rajeshwar D.
Format: Article
Language:English
Subjects:
Biodiesel
Biofuels
Biomass
Box-Behnken design
chloroform
detergents
Detergents - chemistry
Lipid extraction
lipids
Lipids - isolation & purification
Methanol
microbial biomass
Models, Statistical
N-lauroyl sarcosine
response surface methodology
Sarcosine - analogs & derivatives
sarcosines
statistical analysis
technology
Temperature
Yarrowia - chemistry
Yarrowia lipolytica
yeasts
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Summary:•Wet oleaginous yeast biomass was used for lipid extraction.•Biodegradable anionic detergent (N-lauroyl sarcosine) was used for cell disruption.•N-lauroyl sarcosine minimized lipid extraction time.•N-lauroyl sarcosine had no effect on fatty acid profiles. The lipid extraction from the microbial biomass is a tedious and high cost dependent process. In the present study, detergent assisted lipids extraction from the culture of the yeast Yarrowia lipolytica SKY-7 was carried out. Response surface methodology (RSM) was used to investigate the effect of three principle parameters (N-LS concentration, time and temperature) on microbial lipid extraction efficiency % (w/w). The results obtained by statistical analysis showed that the quadratic model fits in all cases. Maximum lipid recovery of 95.3±0.3% w/w was obtained at the optimum level of process variables [N-LS concentration 24.42mg (equal to 48mgN-LS/g dry biomass), treatment time 8.8min and reaction temperature 30.2°C]. Whereas the conventional chloroform and methanol extraction to achieve total lipid recovery required 12h at 60°C. The study confirmed that oleaginous yeast biomass treatment with N-lauroyl sarcosine would be a promising approach for industrial scale microbial lipid recovery.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.07.011