Effects of gallic acid on delta – aminolevulinic dehydratase activity and in the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats

• Published in: Biomedicine & pharmacotherapy 2016-12, Vol.84, p.1291-1299
• Main Authors: de Oliveira, Lizielle Souza, Thomé, Gustavo Roberto, Lopes, Thauan Faccin, Reichert, Karine Paula, de Oliveira, Juliana Sorraila, da Silva Pereira, Aline, Baldissareli, Jucimara, da Costa Krewer, Cristina, Morsch, Vera Maria, Chitolina Schetinger, Maria Rosa, Spanevello, Roselia Maria
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Blood Glucose - metabolism; Body Weight - drug effects; Catalase - metabolism; Delta-aminolevulinic acid dehydratase; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - enzymology; Diabetes Mellitus, Experimental - pathology; Gallic acid; Gallic Acid - chemistry; Gallic Acid - pharmacology; Gallic Acid - therapeutic use; Glutathione Transferase - metabolism; Hepatocytes - drug effects; Hepatocytes - pathology; Internal Medicine; Kidney; Kidney - drug effects; Kidney - enzymology; Kidney - pathology; Lipid Peroxidation - drug effects; Liver; Liver - drug effects; Liver - enzymology; Liver - pathology; Male; Medical Education; Oxidative Stress - drug effects; Porphobilinogen Synthase - metabolism; Rats, Wistar; Reactive Oxygen Species - metabolism; Streptozocin; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - metabolism
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2016.10.021

Abstract Diabetes mellitus (DM) is characterised by hyperglycaemia associated with the increase of oxidative stress. Gallic acid has potent antioxidant properties. The aim of this study was to evaluate the effect of gallic acid on the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats. Male rats were divided in groups: control, gallic acid, diabetic and diabetic plus gallic acid. DM was induced in the animals by intraperitoneal injection of streptozotocin (65 mg/kg). Gallic acid (30 mg/kg) was administered orally for 21 days. Our results showed an increase in reactive species levels and lipid peroxidation, and a decrease in activity of the enzymes superoxide dismutase and delta-aminolevulinic acid dehydratase in the liver and kidney of the diabetic animals (P < 0.05). Gallic acid treatment showed protective effects in these parameters evaluated, and also prevented a decrease in the activity of catalase and glutathione S-transferase, and vitamin C levels in the liver of diabetic rats. In addition, gallic acid reduced the number of nuclei and increased the area of the core in hepatic tissue, and increased the glomerular area in renal tissue. These results indicate that gallic acid can protect against oxidative stress-induced damage in the diabetic state.