Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

Abstract A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was ge...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2016-10, Vol.497, p.111-124
Main Authors: Chen, Zhenhai, Yuan, Fangfeng, Li, Yanhua, Shang, Pengcheng, Schroeder, Robin, Lechtenberg, Kelly, Henningson, Jamie, Hause, Benjamin, Bai, Jianfa, Rowland, Raymond R.R, Clavijo, Alfonso, Fang, Ying
Format: Article
Language:English
Subjects:
Adaptive Immunity
Animals
Antibodies, Viral - immunology
Base Sequence
Cell Line
Cloning, Molecular
Cytokines - metabolism
DNA, Complementary - chemistry
DNA, Complementary - genetics
EGFP reporter virus
Gene Expression
Genes, Reporter
Genetic Vectors - genetics
Genome, Viral
Immunity, Innate
Infectious clone
Infectious Disease
Mutation
Phenotype
Picornaviridae - genetics
Picornaviridae - immunology
Picornaviridae Infections - veterinary
Picornavirus infection
Recombination, Genetic
Reverse genetics
RNA, Viral
Senecavirus A
SVA pathogenesis
Swine
Swine Diseases - diagnosis
Swine Diseases - immunology
Swine Diseases - metabolism
Swine Diseases - virology
Vesicular disease
Vesicular lesion
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Summary:Abstract A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2016.07.003