Physiological, biochemical, and proteome profiling reveals key pathways underlying the drought stress responses of Hippophae rhamnoides

• Published in: Proteomics (Weinheim) 2016-10, Vol.16 (20), p.2688-2697
• Main Authors: He, Cai Y., Zhang, Guo Y., Zhang, Jian G., Duan, Ai G., Luo, Hong M.
• Format: Article
• Language: English
• Subjects: Abscisic acid; Acetic acid; Adaptation, Physiological; Adaptations; Agricultural production; Climate change; DIGE; Drought; Drought stress; Droughts; Epigenesis, Genetic; Epigenetic modification / Hippophae rhamnoides; Epigenetics; Gene Expression Regulation, Plant; Glutathione; Glutathione reductase; Hippophae - genetics; Hippophae - physiology; Hippophae rhamnoides; Indoleacetic acid; Leaves; Malondialdehyde; Membrane conductance; Membrane permeability; Metabolism; Photosynthesis; Physiology; Plant growth; Plant Proteins - analysis; Plant Proteins - genetics; Plant Proteins - metabolism; Plant proteomics; Proteome profiling; Proteomes; Proteomics; Resistance; RNA, Messenger - genetics; Roots; Seedlings; Signal Transduction; Soil conservation; Soil permeability; Soil water; Stomata; Stomatal conductance; Stress, Physiological; Superoxide dismutase; Transpiration; Water conservation; Water potential; Water resources; Water scarcity; Water shortages; Water use; Water use efficiency; Zeatin; Zeatin riboside
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201600160

The effects of drought on plant growth and development are occurring as a result of climate change and the growing scarcity of water resources. Hippophae rhamnoides has been exploited for soil and water conservation for many years. However, the outstanding drought‐resistance mechanisms possessed by this species remain unclear. The protein, physiological, and biochemical responses to medium and severe drought stresses in H. rhamnoides seedlings are analyzed. Linear decreases in photosynthesis rate, transpiration rate, and the content of indole acetic acid in roots, as well as a linear increase in the contents of abscisic acid, superoxide dismutase, glutathione reductase, and zeatin riboside in leaves are observed as water potential decreased. At the same time, cell membrane permeability, malondialdehyde, stomatal conductance, water use efficiency, and contents of zeatin riboside in roots and indole acetic acid in leaves showed nonconsistent changes. DIGE and MS/MS analysis identified 51 differently expressed protein spots in leaves with functions related to epigenetic modification and PTM in addition to normal metabolism, photosynthesis, signal transduction, antioxidative systems, and responses to stimuli. This study provides new insights into the responses and adaptations in this drought‐resistant species and may benefit future agricultural production.