Ectopic bone formation during tissue-engineered cartilage repair using autologous chondrocytes and novel plasma-derived albumin scaffolds

Summary Purpose The aims of this study were twofold: first, to evaluate the production of cartilaginous tissue in vitro and in vivo using a novel plasma-derived scaffold, and second, to test the repair of experimental defects made on ears of New Zealand rabbits (NZr) using this approach. Materials a...

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Bibliographic Details
Published in:Journal of cranio-maxillo-facial surgery 2016-10, Vol.44 (10), p.1743-1749
Main Authors: Robla Costales, David, MD, Junquera, Luis, MD, PhD, García Pérez, Eva, Gómez Llames, Sara, Álvarez-Viejo, María, Meana-Infiesta, Álvaro, MD, PhD
Format: Article
Language:English
Subjects:
Albumin
Animals
Cartilage
Cartilage - growth & development
Cartilage - injuries
Chondrocyte
Chondrocytes - physiology
Chondrocytes - transplantation
Dentistry
Ear Cartilage - growth & development
Ear Cartilage - injuries
In Vitro Techniques
Mice
Mice, Nude
Ossification
Ossification, Heterotopic - prevention & control
Rabbits
Scaffold
Surgery
Tissue Engineering - methods
Tissue Scaffolds
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Summary:Summary Purpose The aims of this study were twofold: first, to evaluate the production of cartilaginous tissue in vitro and in vivo using a novel plasma-derived scaffold, and second, to test the repair of experimental defects made on ears of New Zealand rabbits (NZr) using this approach. Materials and Methods Scaffolds were seeded with chondrocytes and cultured in vitro for 3 months to check in vitro cartilage production. To evaluate in vivo cartilage production, a chondrocyte-seeded scaffold was transplanted subcutaneously to a nude mouse. To check in vivo repair, experimental defects made in the ears of five New Zealand rabbits (NZr) were filled with chondrocyte-seeded scaffolds. Results In vitro culture produced mature chondrocytes with no extracellular matrix (ECM). Histological examination of redifferentiated in vitro cultures showed differentiated chondrocytes adhered to scaffold pores. Subcutaneous transplantation of these constructs to a nude mouse produced cartilage, confirmed by histological study. Experimental cartilage repair in five NZr showed cartilaginous tissue repairing the defects, mixed with calcified areas of bone formation. Conclusion It is possible to produce cartilaginous tissue in vivo and to repair experimental auricular defects by means of chondrocyte cultures and the novel plasma-derived scaffold. Further studies are needed to determine the significance of bone formation in the samples.
ISSN:1010-5182
1878-4119
DOI:10.1016/j.jcms.2016.08.005