The appressorium of the rice blast fungus Magnaporthe oryzae remains mitotically active during post-penetration hyphal growth

•M. oryzae appressoria remain mitotically active during IH proliferation.•Sister chromatids separate within the appressorium.•A mitotic nucleus undergoes extreme constriction and elongation through the penetration peg. To investigate the mitotic dynamics of an appressorium, we used live-cell confoca...

Full description

Saved in:
Bibliographic Details
Published in:Fungal genetics and biology 2017-01, Vol.98, p.35-38
Main Authors: Jenkinson, Cory B., Jones, Kiersun, Zhu, Jie, Dorhmi, Sara, Khang, Chang Hyun
Format: Article
Language:English
Subjects:
Appressoria
Cell Nucleus
Fungal Proteins - genetics
Hyphae - genetics
Hyphae - growth & development
Hyphae - pathogenicity
Magnaporthe - genetics
Magnaporthe - growth & development
Magnaporthe - pathogenicity
Mitosis
Mitosis - genetics
Nuclear dynamics
Oryza - microbiology
Plant Diseases - genetics
Plant Diseases - microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•M. oryzae appressoria remain mitotically active during IH proliferation.•Sister chromatids separate within the appressorium.•A mitotic nucleus undergoes extreme constriction and elongation through the penetration peg. To investigate the mitotic dynamics of an appressorium, we used live-cell confocal imaging of a fluorescence-based mitotic reporter strain of Magnaporthe oryzae. We present evidence that the M. oryzae appressorium remains viable and mitotically active well after host penetration. These results suggest the potential roles of the appressorium during post-penetration proliferation of invasive hyphae. Our studies also revealed that a mitotic appressorial nucleus undergoes extreme constriction and elongation as it migrates through the penetration peg in a manner analogous to mitosis during cell-to-cell movement of invasive hyphae. Understanding the mechanisms underlying these pathogen-specific nuclear dynamics may provide new targets for disease control.
ISSN:1087-1845
1096-0937
DOI:10.1016/j.fgb.2016.11.006