CEA-targeted nanoparticles allow specific in vivo fluorescent imaging of colorectal cancer models

Fluorescent imaging of colorectal tumor cells would improve tumor localization and allow intra-operative staging, facilitating stratification of surgical resections thereby improving patient outcomes. We aimed to develop and test fluorescent nanoparticles capable of allowing this in vivo. Dye-doped...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2015-04, Vol.10 (8), p.1223-1231
Main Authors: Tiernan, James P, Ingram, Nicola, Marston, Gemma, Perry, Sarah L, Rushworth, Jo V, Coletta, P Louise, Millner, Paul A, Jayne, David G, Hughes, Thomas A
Format: Article
Language:English
Subjects:
Analysis
Animals
Carcinoembryonic Antigen - analysis
Care and treatment
Cell Line, Tumor
Colon - pathology
Colorectal cancer
Colorectal Neoplasms - pathology
Diagnosis
Female
Fluorescence
Fluorescent Dyes - chemistry
Humans
Immunoconjugates - chemistry
Laparoscopy
Measurement
Mice, Inbred BALB C
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Optical Imaging - methods
Rectum - pathology
Silicon Dioxide - chemistry
Wavelength
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Summary:Fluorescent imaging of colorectal tumor cells would improve tumor localization and allow intra-operative staging, facilitating stratification of surgical resections thereby improving patient outcomes. We aimed to develop and test fluorescent nanoparticles capable of allowing this in vivo. Dye-doped silica nanoparticles were synthesized. Anti-CEA (carcinoembryonic antigen) or control IgGs were conjugated to nanoparticles using various chemical strategies. Binding of CEA-targeted or control nanoparticles to colorectal cancer cells was quantified in vitro, and in vivo after systemic-delivery to murine xenografts. CEA-targeted, polyamidoamine dendrimer-conjugated, nanoparticles, but not control nanoparticles, allowed strong tumor-specific imaging. We are the first to demonstrate live, specific, in vivo imaging of colorectal cancer cells using antibody-targeted fluorescent nanoparticles. These nanoparticles have potential to allow intra-operative fluorescent visualization of tumor cells.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm.14.202