Digital gene expression profiling analysis of DNA repair pathways in colon cancer stem population of HT29 cells

• Published in: Acta biochimica et biophysica Sinica 2017, Vol.49 (1), p.90-100
• Main Authors: Wang, Wenxue, Zhang, Guoxiu, Yang, Jing, Gu, Huan, Ding, Lei, Yu, Haijing, Yu, Min, Cui, Qinghua, Ji, Xinglai, Li, Meizhang
• Format: Article
• Language: English
• Subjects: Colonic Neoplasms - genetics; Colonic Neoplasms - pathology; DNA Repair - genetics; DNA修复基因; Gene Expression Profiling; HT29 Cells; Humans; Neoplastic Stem Cells - metabolism; 人口; 基因表达分析; 细胞数; 结肠癌; 肿瘤干细胞; 表达谱分析; 非同源末端连接
• ISSN: 1672-9145; 1745-7270
• DOI: 10.1093/abbs/gmw119

Cancer stem cells （CSCs） contribute to the relapse and development of new neoplasm lesions. While most available clinical approaches, such as chemical and radiation therapies, will kill the majority of cancer cells, they do not kill them all. Some resisting cells, like CSCs, are able to sur- vive due to their excellent self-maintaining capabilities, even in challenging environments. In the present study, we investigated the mRNA level of DNA repair genes of colon CSCs from the HT29 cell line in response to single-strand damage and double-strand breaks, as well as the evident upregulation of key genes in base excision repair, mismatch repair, non-homologous end-joining, and homologous recombination pathways in these cells. Digital gene expression analysis identi- fied upregulated genes in CD44＋ HT29 cells that may play important roles in DNA repair. Our results reveal that colon CSCs bear efficient DNA repair abilities, which might explain the survival of colon CSCs after repeated chemical and radiation therapy.