Biophysical Effects of a Polymeric Biosurfactant in Candida krusei and Candida albicans Cells

• Published in: Mycopathologia (1975) 2016-12, Vol.181 (11-12), p.799-806
• Main Authors: Ferreira, Gabriella Freitas, dos Santos Pinto, Bruna Lorrana, Souza, Eliene Batista, Viana, José Lima, Zagmignan, Adrielle, dos Santos, Julliana Ribeiro Alves, Santos, Áquila Rodrigues Costa, Tavares, Priscila Batista, Denadai, Ângelo Márcio Leite, Monteiro, Andrea Souza
• Format: Article
• Language: English
• Subjects: Antifungal Agents - pharmacology; Biofilms - drug effects; Biomedical and Life Sciences; Biophysical Phenomena - drug effects; Biopolymers - pharmacology; Candida - drug effects; Candida - physiology; Candida albicans; Candida krusei; Cell Adhesion - drug effects; Cells, Cultured; Epithelial Cells - microbiology; Eukaryotic Microbiology; Healthy Volunteers; Humans; Life Sciences; Medical Microbiology; Microbial Ecology; Microbiology; Plant Sciences; Surface-Active Agents - isolation & purification; Surface-Active Agents - pharmacology; Surfactants; Trichosporon; Trichosporon - metabolism; Yeast
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1007/s11046-016-0054-z

This study evaluated the effects of a polymeric biosurfactant produced by Trichosporon montevideense CLOA72 in the adhesion of Candida albicans and Candida krusei cells to human buccal epithelial cells and its interference in biofilm formation by these strains. The biofilm inhibition by biosurfactant (25 mg/mL) in C. krusei and C. albicans in polystyrene was reduced up to 79.5 and 85 %, respectively. In addition, the zeta potential and hydrodynamic diameter of the yeasts altered as a function of the biosurfactant concentration added to the cell suspension. The changes in the cell surface characteristics and the interface modification can contribute to the inhibition of the initial adherence of yeasts cells to the surface. In addition, the analyses of the biofilm matrix and planktonic cell surfaces demonstrated differences in carbohydrate and protein concentrations for the two studied strains, which may contribute to the modulation of cell adhesion or consolidation of biofilms, especially in C. krusei . This study suggests a possible application of the of CLOA72 biosurfactant in inhibiting the adhesion and formation of biofilms on biological surfaces by yeasts of the Candida genus.