Nanoparticle passage through porcine jejunal mucus: microfluidics and rheology

Abstract A micro-slide chamber was used to screen and rank sixteen functionalized fluorescent silica nanoparticles (SiNP) of different sizes (10, 50, 100 and 200 nm) and surface coatings (aminated, carboxylated, methyl-PEG1000 ylated, and methyl-PEG2000 ylated) according to their capacity to permeat...

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Bibliographic Details
Published in:Nanomedicine 2017-04, Vol.13 (3), p.863-873
Main Authors: Bhattacharjee, Sourav, Mahon, Eugene, Harrison, Sabine M, McGetrick, Jim, Muniyappa, Mohankumar, Carrington, Stephen D, Brayden, David J
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Drug Carriers - analysis
Drug Carriers - pharmacokinetics
Equipment Design
ImageJ
Internal Medicine
Jejunum - metabolism
Microfluidic Analytical Techniques - instrumentation
Mucodiffusion
Mucus - metabolism
Mucus rheology
Nano-DDS
Nanoparticles - analysis
Nanoparticles - ultrastructure
Oral drug delivery
Polyethylene Glycols - analysis
Polyethylene Glycols - pharmacokinetics
Rheology
Silica nanoparticles
Silicon Dioxide - analysis
Silicon Dioxide - pharmacokinetics
Surface Properties
Swine
Viscosity
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Summary:Abstract A micro-slide chamber was used to screen and rank sixteen functionalized fluorescent silica nanoparticles (SiNP) of different sizes (10, 50, 100 and 200 nm) and surface coatings (aminated, carboxylated, methyl-PEG1000 ylated, and methyl-PEG2000 ylated) according to their capacity to permeate porcine jejunal mucus. Variables investigated were influence of particle size, surface charge and methyl-PEGylation. The anionic SiNP showed higher transport through mucus whereas the cationic SiNP exhibited higher binding with lower transport. A size-dependence in transport was identified – 10 and 50 nm anionic (uncoated or methyl-PEGylated) SiNP showed higher transport compared to the larger 100 and 200 nm SiNP. The cationic SiNP of all sizes interacted with the mucus, making it more viscous and less capable of swelling. In contrast, the anionic SiNP (uncoated or methyl-PEGylated) caused minimal changes in the viscoelasticity of mucus. The data provide insights into mucus-NP interactions and suggest a rationale for designing oral nanomedicines with improved mucopermeability.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2016.11.017