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Allogenic cultivated limbal stem cell transplantation versus cadaveric keratolimbal allograft in ocular surface disorder: 1-year outcome
Purpose To compare the outcome of allogenic ex vivo cultivated limbal stem cell transplantation (LSCT) versus cadaveric keratolimbal allograft in patients with limbal stem cell deficiency (LSCD). Methods In this prospective interventional study from Army Hospital Research and Referral in New Delhi,...
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| Published in: | International ophthalmology 2017-12, Vol.37 (6), p.1323-1331 |
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| container_title | International ophthalmology |
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| creator | Parihar, Jitendra Kumar Singh Parihar, Avinash Singh Jain, Vaibhav Kumar Kaushik, Jaya Nath, Pramod |
| description | Purpose
To compare the outcome of allogenic ex vivo cultivated limbal stem cell transplantation (LSCT) versus cadaveric keratolimbal allograft in patients with limbal stem cell deficiency (LSCD).
Methods
In this prospective interventional study from Army Hospital Research and Referral in New Delhi, 50 eyes of 40 patients with ocular surface disorder having LSCD underwent either cultivated LSCT (group 1: 25 eyes of 20 patients) or keratolimbal allograft transplantation (group 2: 25 eyes of 20 patients). The outcome measures were gain of two or more lines of best-corrected visual acuity (BCVA), absence of conjunctival vascularization over cornea, corneal neovascularization regression, regression of visually significant corneal opacity (iris details poorly visible), Schirmer’s 1 ≥10 s, tear break-up time (TBUT) ≥10 s, and absence of fluorescein staining.
Results
At 1 year of follow-up, both groups experienced significant improvement in all outcome measures, but there was no significant difference between both groups in improvement of two or more lines of BCVA (
P
= 0.841), absence of conjunctival vascularization over cornea (
P
= 0.812), corneal neovascularization (
P
= 0.074), visually significant corneal opacity (
P
= 0.075), Schirmer’s 1 ≥10 s (
P
= 0.814), TBUT ≥10 s (
P
= 0.382), or absence of fluorescein stain (
P
= 1.00).
Conclusion
Both allogenic ex vivo cultivated LSCT and cadaveric keratolimbal allograft transplantation are comparable in terms of visual gain and ocular surface restoration. |
| doi_str_mv | 10.1007/s10792-016-0415-0 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1853359544</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1957706607</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-a05fe952581b359a9d2895554efce92377c5a8cd66d18eb82870b820f89043983</originalsourceid><addsrcrecordid>eNp1kcFqHSEUhqW0NDdJH6CbInTTjc1Rx1G7C6FNC4FumrV4nTNhUme8VSeQN8hjx8u9LaXQjQrn-z-Vn5C3HD5yAH1ROGgrGPCeQccVgxdkw5WWTPQSXpJNGyimNPATclrKPQBYbfvX5EQYEEpbuSFPlzGmO1ymQMMa6_TgKw40TvPWR1oqzjRgjLRmv5Rd9Ev1dUoLfcBc1kKDH3w7tvBPzL6mY87vndmPlU4LTc3rMy1rHn1AOkwl5QHzJ8rZI7ZBWmtIM56TV6OPBd8c9zNy--Xzj6uv7Ob79beryxsWOmkq86BGtEoow7dSWW8HYaxSqsMxoBVS66C8CUPfD9zg1gijoa0wGgudtEaekQ8H7y6nXyuW6uap7P_oF0xrcdwo2cSq6xr6_h_0Pq15aa9z3Cqtoe9BN4ofqJBTKRlHt8vT7POj4-D2NblDTa614fY1OWiZd0fzup1x-JP43UsDxAEobbTcYf7r6v9anwHg-p67</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1957706607</pqid></control><display><type>article</type><title>Allogenic cultivated limbal stem cell transplantation versus cadaveric keratolimbal allograft in ocular surface disorder: 1-year outcome</title><source>Springer Nature</source><creator>Parihar, Jitendra Kumar Singh ; Parihar, Avinash Singh ; Jain, Vaibhav Kumar ; Kaushik, Jaya ; Nath, Pramod</creator><creatorcontrib>Parihar, Jitendra Kumar Singh ; Parihar, Avinash Singh ; Jain, Vaibhav Kumar ; Kaushik, Jaya ; Nath, Pramod</creatorcontrib><description>Purpose
To compare the outcome of allogenic ex vivo cultivated limbal stem cell transplantation (LSCT) versus cadaveric keratolimbal allograft in patients with limbal stem cell deficiency (LSCD).
Methods
In this prospective interventional study from Army Hospital Research and Referral in New Delhi, 50 eyes of 40 patients with ocular surface disorder having LSCD underwent either cultivated LSCT (group 1: 25 eyes of 20 patients) or keratolimbal allograft transplantation (group 2: 25 eyes of 20 patients). The outcome measures were gain of two or more lines of best-corrected visual acuity (BCVA), absence of conjunctival vascularization over cornea, corneal neovascularization regression, regression of visually significant corneal opacity (iris details poorly visible), Schirmer’s 1 ≥10 s, tear break-up time (TBUT) ≥10 s, and absence of fluorescein staining.
Results
At 1 year of follow-up, both groups experienced significant improvement in all outcome measures, but there was no significant difference between both groups in improvement of two or more lines of BCVA (
P
= 0.841), absence of conjunctival vascularization over cornea (
P
= 0.812), corneal neovascularization (
P
= 0.074), visually significant corneal opacity (
P
= 0.075), Schirmer’s 1 ≥10 s (
P
= 0.814), TBUT ≥10 s (
P
= 0.382), or absence of fluorescein stain (
P
= 1.00).
Conclusion
Both allogenic ex vivo cultivated LSCT and cadaveric keratolimbal allograft transplantation are comparable in terms of visual gain and ocular surface restoration.</description><identifier>ISSN: 0165-5701</identifier><identifier>EISSN: 1573-2630</identifier><identifier>DOI: 10.1007/s10792-016-0415-0</identifier><identifier>PMID: 28025793</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acuity ; Adult ; Aged ; Army ; Cadavers ; Cornea ; Corneal Diseases - pathology ; Corneal Diseases - physiopathology ; Corneal Diseases - surgery ; Corneal Neovascularization - pathology ; Corneal Transplantation - methods ; Epithelium, Corneal - transplantation ; Eye (anatomy) ; Female ; Fluorescein ; Humans ; Limbus Corneae - cytology ; Limbus Corneae - surgery ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Opacity ; Ophthalmology ; Original Paper ; Patients ; Postoperative Complications ; Prospective Studies ; Restoration ; Stem cell transplantation ; Stem Cell Transplantation - methods ; Stem cells ; Tears - metabolism ; Transplantation ; Transplantation, Homologous ; Vascularization ; Visual acuity ; Visual Acuity - physiology</subject><ispartof>International ophthalmology, 2017-12, Vol.37 (6), p.1323-1331</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><rights>International Ophthalmology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-a05fe952581b359a9d2895554efce92377c5a8cd66d18eb82870b820f89043983</citedby><cites>FETCH-LOGICAL-c438t-a05fe952581b359a9d2895554efce92377c5a8cd66d18eb82870b820f89043983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28025793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parihar, Jitendra Kumar Singh</creatorcontrib><creatorcontrib>Parihar, Avinash Singh</creatorcontrib><creatorcontrib>Jain, Vaibhav Kumar</creatorcontrib><creatorcontrib>Kaushik, Jaya</creatorcontrib><creatorcontrib>Nath, Pramod</creatorcontrib><title>Allogenic cultivated limbal stem cell transplantation versus cadaveric keratolimbal allograft in ocular surface disorder: 1-year outcome</title><title>International ophthalmology</title><addtitle>Int Ophthalmol</addtitle><addtitle>Int Ophthalmol</addtitle><description>Purpose
To compare the outcome of allogenic ex vivo cultivated limbal stem cell transplantation (LSCT) versus cadaveric keratolimbal allograft in patients with limbal stem cell deficiency (LSCD).
Methods
In this prospective interventional study from Army Hospital Research and Referral in New Delhi, 50 eyes of 40 patients with ocular surface disorder having LSCD underwent either cultivated LSCT (group 1: 25 eyes of 20 patients) or keratolimbal allograft transplantation (group 2: 25 eyes of 20 patients). The outcome measures were gain of two or more lines of best-corrected visual acuity (BCVA), absence of conjunctival vascularization over cornea, corneal neovascularization regression, regression of visually significant corneal opacity (iris details poorly visible), Schirmer’s 1 ≥10 s, tear break-up time (TBUT) ≥10 s, and absence of fluorescein staining.
Results
At 1 year of follow-up, both groups experienced significant improvement in all outcome measures, but there was no significant difference between both groups in improvement of two or more lines of BCVA (
P
= 0.841), absence of conjunctival vascularization over cornea (
P
= 0.812), corneal neovascularization (
P
= 0.074), visually significant corneal opacity (
P
= 0.075), Schirmer’s 1 ≥10 s (
P
= 0.814), TBUT ≥10 s (
P
= 0.382), or absence of fluorescein stain (
P
= 1.00).
Conclusion
Both allogenic ex vivo cultivated LSCT and cadaveric keratolimbal allograft transplantation are comparable in terms of visual gain and ocular surface restoration.</description><subject>Acuity</subject><subject>Adult</subject><subject>Aged</subject><subject>Army</subject><subject>Cadavers</subject><subject>Cornea</subject><subject>Corneal Diseases - pathology</subject><subject>Corneal Diseases - physiopathology</subject><subject>Corneal Diseases - surgery</subject><subject>Corneal Neovascularization - pathology</subject><subject>Corneal Transplantation - methods</subject><subject>Epithelium, Corneal - transplantation</subject><subject>Eye (anatomy)</subject><subject>Female</subject><subject>Fluorescein</subject><subject>Humans</subject><subject>Limbus Corneae - cytology</subject><subject>Limbus Corneae - surgery</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Opacity</subject><subject>Ophthalmology</subject><subject>Original Paper</subject><subject>Patients</subject><subject>Postoperative Complications</subject><subject>Prospective Studies</subject><subject>Restoration</subject><subject>Stem cell transplantation</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem cells</subject><subject>Tears - metabolism</subject><subject>Transplantation</subject><subject>Transplantation, Homologous</subject><subject>Vascularization</subject><subject>Visual acuity</subject><subject>Visual Acuity - physiology</subject><issn>0165-5701</issn><issn>1573-2630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kcFqHSEUhqW0NDdJH6CbInTTjc1Rx1G7C6FNC4FumrV4nTNhUme8VSeQN8hjx8u9LaXQjQrn-z-Vn5C3HD5yAH1ROGgrGPCeQccVgxdkw5WWTPQSXpJNGyimNPATclrKPQBYbfvX5EQYEEpbuSFPlzGmO1ymQMMa6_TgKw40TvPWR1oqzjRgjLRmv5Rd9Ev1dUoLfcBc1kKDH3w7tvBPzL6mY87vndmPlU4LTc3rMy1rHn1AOkwl5QHzJ8rZI7ZBWmtIM56TV6OPBd8c9zNy--Xzj6uv7Ob79beryxsWOmkq86BGtEoow7dSWW8HYaxSqsMxoBVS66C8CUPfD9zg1gijoa0wGgudtEaekQ8H7y6nXyuW6uap7P_oF0xrcdwo2cSq6xr6_h_0Pq15aa9z3Cqtoe9BN4ofqJBTKRlHt8vT7POj4-D2NblDTa614fY1OWiZd0fzup1x-JP43UsDxAEobbTcYf7r6v9anwHg-p67</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Parihar, Jitendra Kumar Singh</creator><creator>Parihar, Avinash Singh</creator><creator>Jain, Vaibhav Kumar</creator><creator>Kaushik, Jaya</creator><creator>Nath, Pramod</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>Allogenic cultivated limbal stem cell transplantation versus cadaveric keratolimbal allograft in ocular surface disorder: 1-year outcome</title><author>Parihar, Jitendra Kumar Singh ; Parihar, Avinash Singh ; Jain, Vaibhav Kumar ; Kaushik, Jaya ; Nath, Pramod</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-a05fe952581b359a9d2895554efce92377c5a8cd66d18eb82870b820f89043983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acuity</topic><topic>Adult</topic><topic>Aged</topic><topic>Army</topic><topic>Cadavers</topic><topic>Cornea</topic><topic>Corneal Diseases - pathology</topic><topic>Corneal Diseases - physiopathology</topic><topic>Corneal Diseases - surgery</topic><topic>Corneal Neovascularization - pathology</topic><topic>Corneal Transplantation - methods</topic><topic>Epithelium, Corneal - transplantation</topic><topic>Eye (anatomy)</topic><topic>Female</topic><topic>Fluorescein</topic><topic>Humans</topic><topic>Limbus Corneae - cytology</topic><topic>Limbus Corneae - surgery</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Opacity</topic><topic>Ophthalmology</topic><topic>Original Paper</topic><topic>Patients</topic><topic>Postoperative Complications</topic><topic>Prospective Studies</topic><topic>Restoration</topic><topic>Stem cell transplantation</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem cells</topic><topic>Tears - metabolism</topic><topic>Transplantation</topic><topic>Transplantation, Homologous</topic><topic>Vascularization</topic><topic>Visual acuity</topic><topic>Visual Acuity - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parihar, Jitendra Kumar Singh</creatorcontrib><creatorcontrib>Parihar, Avinash Singh</creatorcontrib><creatorcontrib>Jain, Vaibhav Kumar</creatorcontrib><creatorcontrib>Kaushik, Jaya</creatorcontrib><creatorcontrib>Nath, Pramod</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>International ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parihar, Jitendra Kumar Singh</au><au>Parihar, Avinash Singh</au><au>Jain, Vaibhav Kumar</au><au>Kaushik, Jaya</au><au>Nath, Pramod</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Allogenic cultivated limbal stem cell transplantation versus cadaveric keratolimbal allograft in ocular surface disorder: 1-year outcome</atitle><jtitle>International ophthalmology</jtitle><stitle>Int Ophthalmol</stitle><addtitle>Int Ophthalmol</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>37</volume><issue>6</issue><spage>1323</spage><epage>1331</epage><pages>1323-1331</pages><issn>0165-5701</issn><eissn>1573-2630</eissn><abstract>Purpose
To compare the outcome of allogenic ex vivo cultivated limbal stem cell transplantation (LSCT) versus cadaveric keratolimbal allograft in patients with limbal stem cell deficiency (LSCD).
Methods
In this prospective interventional study from Army Hospital Research and Referral in New Delhi, 50 eyes of 40 patients with ocular surface disorder having LSCD underwent either cultivated LSCT (group 1: 25 eyes of 20 patients) or keratolimbal allograft transplantation (group 2: 25 eyes of 20 patients). The outcome measures were gain of two or more lines of best-corrected visual acuity (BCVA), absence of conjunctival vascularization over cornea, corneal neovascularization regression, regression of visually significant corneal opacity (iris details poorly visible), Schirmer’s 1 ≥10 s, tear break-up time (TBUT) ≥10 s, and absence of fluorescein staining.
Results
At 1 year of follow-up, both groups experienced significant improvement in all outcome measures, but there was no significant difference between both groups in improvement of two or more lines of BCVA (
P
= 0.841), absence of conjunctival vascularization over cornea (
P
= 0.812), corneal neovascularization (
P
= 0.074), visually significant corneal opacity (
P
= 0.075), Schirmer’s 1 ≥10 s (
P
= 0.814), TBUT ≥10 s (
P
= 0.382), or absence of fluorescein stain (
P
= 1.00).
Conclusion
Both allogenic ex vivo cultivated LSCT and cadaveric keratolimbal allograft transplantation are comparable in terms of visual gain and ocular surface restoration.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>28025793</pmid><doi>10.1007/s10792-016-0415-0</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| issn | 0165-5701 1573-2630 |
| language | eng |
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| source | Springer Nature |
| subjects | Acuity Adult Aged Army Cadavers Cornea Corneal Diseases - pathology Corneal Diseases - physiopathology Corneal Diseases - surgery Corneal Neovascularization - pathology Corneal Transplantation - methods Epithelium, Corneal - transplantation Eye (anatomy) Female Fluorescein Humans Limbus Corneae - cytology Limbus Corneae - surgery Male Medicine Medicine & Public Health Middle Aged Opacity Ophthalmology Original Paper Patients Postoperative Complications Prospective Studies Restoration Stem cell transplantation Stem Cell Transplantation - methods Stem cells Tears - metabolism Transplantation Transplantation, Homologous Vascularization Visual acuity Visual Acuity - physiology |
| title | Allogenic cultivated limbal stem cell transplantation versus cadaveric keratolimbal allograft in ocular surface disorder: 1-year outcome |
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