enterotoxin T (BcET) from Bacillus cereus can probably not contribute to food poisoning

A polymerase chain reaction (PCR) fragment from strain NVH 38 (containing bceT) was cloned, sequenced and expressed in Escherichia coli. This sequence showed 50-60% identity to the original. When this bceT clone was expressed in E. coli no biological activity was found in either supernatants or cell...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-11, Vol.217 (1), p.115-119
Main Authors: Choma, C, Granum, P.E
Format: Article
Language:English
Subjects:
Animals
Bacillus cereus
Bacillus cereus - genetics
Bacillus cereus - growth & development
Bacteriology
Biological activity
Biological and medical sciences
Cells, Cultured
Cercopithecus aethiops
Cyclic AMP - analysis
Cytotoxicity
E coli
Enterotoxin
Enterotoxins
Enterotoxins - biosynthesis
Enterotoxins - genetics
Enterotoxins - toxicity
Escherichia coli
Food chains
Food contamination
Food Microbiology
Food poisoning
Foodborne Diseases - microbiology
foodborne illness
Fundamental and applied biological sciences. Psychology
Microbiology
Monoclonal antibodies
neutralization
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Polymerase chain reaction
Polymerase Chain Reaction - methods
Vero cell
Vero Cells
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Summary:A polymerase chain reaction (PCR) fragment from strain NVH 38 (containing bceT) was cloned, sequenced and expressed in Escherichia coli. This sequence showed 50-60% identity to the original. When this bceT clone was expressed in E. coli no biological activity was found in either supernatants or cell extracts. Cell extracts from the Bacillus cereus strains (NVH 38 and B-4ac) were also negative on Vero cells. Neutralisation of supernatant from B. cereus B-4ac using a monoclonal antibody (reacting with NheB and HblD) abolished the activity. A test for cytotoxic enterotoxins was negative for both cell extracts and supernatants. Our data suggest that BcET either has an unknown type enterotoxic action or non at all.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11464.x