Adhesive barnacle peptides exhibit a steric-driven design rule to enhance adhesion between asymmetric surfaces

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2017-04, Vol.152, p.42-48
• Main Authors: Raman, Sangeetha, Malms, Lukas, Utzig, Thomas, Shrestha, Buddha Ratna, Stock, Philipp, Krishnan, Shankar, Valtiner, Markus
• Format: Article
• Language: English
• Subjects: Adhesion interface; Adsorption; Barnacle cement; Hydrophobic and Hydrophilic Interactions; Hydrophobic interactions; Non-covalent binding; Peptide bridging; Peptides - chemistry; Surface Properties; Underwater adhesive
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2016.12.038

[Display omitted] •Molecular interaction of two barnacle-inspired peptide sequences is studied.•The peptides asymmetrically bridge with hydrophobic and charged surfaces.•The investigation reveals the necessity of surface charge to promote bridging. Barnacles exhibit superior underwater adhesion simply through sequencing of the 21 proteinogenic amino acids, without post processing or using special amino acids. Here, we measure and discuss the molecular interaction of two distinct and recurring short peptide sequences (Bp1 and Bp2) inspired from the surface binding 19kDa protein from the barnacle attachment interface. Using self-assembled monolayer (SAMs) of known physical and chemical properties on molecularly smooth gold substrates in 5mM NaCl at pH 7.3, (1) the adsorption mechanisms of the barnacle inspired peptides are explored using quartz crystal microbalance, and (2) adhesion mediating properties are measured using the surface force apparatus. The hydrophobic Bp1 peptide with a cysteine residue adsorbs irreversibly onto Au surfaces due to thiol bond formation, while on hydrophobic CH3 SAM surface, the interactions are hydrophobic in nature. Interestingly, Bp2 that contains both hydrophobic and protonated amine units exhibits asymmetric bridging with an exceptionally high adhesion energy up to 100mJ/m2 between mica and both gold and CH3 SAM. Surprisingly on hydrophilic surfaces such as COOH- or OH-SAMs both peptides fail to show any interactions, implying the necessity of surface charge to promote bridging. Our results provide insights into the molecular aspects of manipulating and utilizing barnacle-mediated peptides to promote or inhibit underwater adhesion.