Ionic composition of the rectal contents and excreta of the reduviid bug Triatoma infestans

• Published in: Journal of insect physiology 2001-07, Vol.47 (7), p.739-747
• Main Authors: Kollien, Astrid H, Grospietsch, Thorsten, Kleffmann, Torsten, Zerbst-Boroffka, Irene, Schaub, Günter A
• Format: Article
• Language: English
• Subjects: Excreta; Ions; Osmolality; Reduviidae; Triatoma infestans
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/S0022-1910(00)00170-0

We have investigated the chemical composition of the rectal contents, faeces and urine of the blood-sucking bug Triatoma infestans. This is the environment in which the important disease-causing organism, Trypanosoma cruzi, lives. Directly after feeding of Triatoma infestans, the pH of the excreta switched from an acidic to an alkaline pH and, 1 day later, back to a slightly acidic pH. The osmolality varied in the initial excreta and in the rectal contents on the day following the meal between 300 and 460 mosmol/kg H 2O, but after an additional day it increased to 350–970 mosmol/kg H 2O. Determinations by ion capillary electrophoresis showed that sulphate and phosphate dominated the rectal contents in unfed bugs. After feeding, the first four drops of fluid excreta were mainly a sodium chloride solution (>150 mM for each). One to 10 days after feeding strong individual variations in the concentrations of individual ions were evident, especially for potassium and sodium. Mean concentrations of chloride remained at about 70 mM; sulphate and phosphate showed an increase within the first 1 or 2 days and then reached a level of about 160 and 210 mM, respectively. The rectal contents of long-term starved bugs contained high concentrations of phosphate and potassium; sulphate and sodium were slightly lower.